AMADA INSTRUMENT CO.,LIMITED — Nanjing China
Microscope Slide

Improved Neubauer Hemocytometer Blood Cell Counting Chambers

The Improved Neubauer Hemocytometer is a reusable glass counting chamber designed for precise cell density measurements in blood, bacteria, and fungal spore samples.

Product Overview

The Improved Neubauer Hemocytometer is a reusable glass counting chamber designed for precise cell density measurements in blood, bacteria, and fungal spore samples. It features a 0.100 mm cell depth and a 0.1 microliter volume per counting area, with a grid pattern calibrated to 1/400 square mm for consistent counting across the central 1 mm2 field. The chamber is made from medical grade glass with high light transmission and clear visibility, enabling accurate visual analysis under a microscope.

  • Cell depth0.100 mm
  • Volume per counting area0.1 Microliter
  • Ruling patternImproved Neubauer, 1/400 Square mm
  • MaterialMedical grade glass
  • ReusabilityYes
  • Surface finishSmooth, flat

Key Benefits

  • Reusable design reduces cost per test compared to disposable chambers
  • 0.100 mm cell depth ensures consistent volume for accurate cell count calculations
  • 0.1 microliter volume per counting area matches standard hemocytometer protocols
  • Improved Neubauer grid pattern with 1/400 square mm rulings allows precise counting in defined fields
  • Medical grade glass provides high clarity and light transmission for sharp microscopic visualization

Application Scenarios

  • Counting leukocytes, erythrocytes, and thrombocytes in clinical blood samples
  • Measuring bacterial or fungal spore concentrations in microbiological cultures
  • Quality control checks in pharmaceutical or biotech labs for cell suspension consistency
  • Teaching laboratory sessions on cell counting techniques in biology and medical training
  • Research applications requiring repeatable cell density measurements in small sample volumes

Who It Is For

  • Clinical and veterinary laboratories performing blood cell analysis
  • Microbiology labs testing bacterial and fungal cultures
  • University and school teaching labs for biology and medical science instruction
  • Pharmaceutical and biotechnology quality control teams
  • Research labs conducting cell culture or suspension studies

Problems It Solves

  • Inconsistent cell depth in low-cost chambers leads to inaccurate cell counts; this chamber maintains a fixed 0.100 mm depth
  • Poor glass clarity or light transmission makes it difficult to distinguish cells under the microscope
  • Non-standard grid patterns require manual calibration; the Improved Neubauer pattern is pre-calibrated to 1/400 square mm
  • Disposable chambers increase long-term costs; this reusable version reduces per-use expense
  • Lack of volume standardization across chambers causes variability in results; this chamber uses a fixed 0.1 microliter volume

Technical Features

  • Reusability: designed for cleaning and repeated use without degradation
  • Cell depth: 0.100 mm, standard for hemocytometer-based cell counting
  • Volume per counting area: 0.1 microliter, consistent with standard protocols
  • Grid pattern: Improved Neubauer, with 1/400 square mm rulings for precise field division
  • Material: Medical grade glass with high light transmission and optical clarity
  • Surface finish: Smooth, flat surface to prevent cell trapping or uneven flow
  • Dimensions: Standard microscope slide size, compatible with most upright and inverted microscopes

Specifications

Cell depth0.100 mm
Volume per counting area0.1 Microliter
Ruling patternImproved Neubauer, 1/400 Square mm
MaterialMedical grade glass
ReusabilityYes
Surface finishSmooth, flat
CompatibilityStandard microscope slide size, fits upright and inverted microscopes

Specifications are taken from the AMADA product data for this item. Confirm critical dimensions with us in writing before ordering.

Customization Options

  • Available in standard size only; no custom grid patterns or dimensions provided
  • No option for different cell depths or volumes beyond the standard 0.100 mm and 0.1 microliter
  • No custom labeling or marking available on the glass surface

Comparison With Other Options

Standard Neubauer hemocytometerThe Improved Neubauer pattern has enhanced grid clarity and spacing, reducing counting errors in high-density samples
Disposable plastic hemocytometersThis glass version is reusable and maintains consistent depth and optical quality over time; plastic versions degrade with cleaning and may warp
Non-standard counting chambersThis chamber uses a calibrated 1/400 square mm pattern and 0.1 microliter volume, ensuring compatibility with established counting protocols

Use Guide

  • Clean the chamber with distilled water and dry thoroughly before first use
  • Place a coverslip over the chamber before loading the sample to ensure even cell distribution
  • Load sample using a pipette; avoid overfilling or introducing air bubbles
  • Count cells in the central 1 mm2 area using the Improved Neubauer grid pattern
  • Use the formula: (Total cells counted) x (Dilution factor) x (10,000) = cells per microliter
Read Next

Guides and Applications

Answers

Frequently Asked Questions

What is the cell depth of this hemocytometer?

0.100 mm. This depth is standard for accurate cell count calculations.

What is the volume of the counting area?

0.1 microliter per counting area, consistent with standard hemocytometer protocols.

Can this chamber be reused after cleaning?

Yes. It is designed for repeated use after proper cleaning and drying.

Is the grid pattern the same as a standard Neubauer?

No. It uses an Improved Neubauer pattern with enhanced grid clarity and spacing for more accurate counting.

What type of glass is used?

Medical grade glass with high light transmission and optical clarity.

Can I use this with a microscope that has a 40X objective?

Yes. The chamber is compatible with standard microscope objectives, including 40X.

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