AMADA INSTRUMENT CO.,LIMITED — Nanjing China
Microscope Objective

Plan Infinite/Finite Biologocal Phase Contrast Objective

The Plan Infinite/Finite Biological Phase Contrast Objectives are designed for use in biological microscopy with phase contrast illumination.

Product Overview

The Plan Infinite/Finite Biological Phase Contrast Objectives are designed for use in biological microscopy with phase contrast illumination. They are available in magnifications from 4X to 100X, with numerical apertures from 0.1 to 1.25, and are supplied with a 45mm parfocal length. The infinite and finite series differ in working distance and field of view, allowing selection based on microscope tube length and specimen clearance needs.

  • ModelPlan Infinite Biological Phase Contrast
  • Magnification4XPH, 10XPH, 20XPH, 40XPH, 100XPH
  • Numerical Aperture0.1, 0.25, 0.45, 0.65, 1.25
  • Working Distance16.15mm (4X), 9.57mm (10X), 2.8mm (20X), 0.55mm (40X), 0.21mm (100X)
  • Field of View25mm
  • Field Number25

Key Benefits

  • 4X to 100X magnification range covers routine staining to high-resolution cell and tissue analysis
  • Numerical aperture up to 1.25 enables high resolution and contrast in transparent specimens
  • 45mm parfocal length ensures consistent focus when switching between objectives
  • Infinite and finite series available to match microscope tube length and optical design
  • 25mm and 22mm field of view options provide different image coverage for slide size and inspection area

Application Scenarios

  • Routine inspection of unstained cell cultures and live tissue samples under phase contrast
  • High-magnification analysis of cellular structures and subcellular details at 40X and 100X
  • Quality control in biological labs where consistent focus and contrast are required across multiple samples
  • Teaching labs requiring interchangeable objectives with known parfocal distance for student use
  • Research applications needing precise optical performance from 4X to 100X with minimal spherical and chromatic aberration

Who It Is For

  • Clinical and veterinary laboratories performing cell and tissue analysis
  • University and school teaching labs in biology and life sciences
  • Research labs conducting phase contrast microscopy on live or unstained specimens
  • Quality control departments in biotech and pharmaceutical production
  • Microscopy users working with transparent or low-contrast biological samples

Problems It Solves

  • Inconsistent focus when switching objectives due to non-parfocal design
  • Limited contrast in transparent biological specimens without phase contrast optics
  • Mismatch between objective tube length and microscope optical system due to incorrect infinite/finite design
  • Inadequate working distance for handling slides or specimens under high magnification
  • Field of view too small or too large for standard slide dimensions or inspection area

Technical Features

  • Plan optical design reduces spherical and chromatic aberration across the field
  • Phase contrast rings for 4X, 10X, 20X, 40X and 100X magnifications enable contrast enhancement in transparent samples
  • Infinite and finite series available with different working distances and field of view
  • 45mm parfocal length ensures focus remains stable when changing objectives
  • Numerical aperture values from 0.1 to 1.25 support resolution and light gathering across magnification levels

Specifications

ModelPlan Infinite Biological Phase Contrast
Magnification4XPH, 10XPH, 20XPH, 40XPH, 100XPH
Numerical Aperture0.1, 0.25, 0.45, 0.65, 1.25
Working Distance16.15mm (4X), 9.57mm (10X), 2.8mm (20X), 0.55mm (40X), 0.21mm (100X)
Field of View25mm
Field Number25
Parfocal Length45mm
Optical SystemInfinite
ModelPlan Finite Biological Phase Contrast
Magnification4XPH, 10XPH, 20XPH, 40XPH, 100XPH
Numerical Aperture0.1, 0.25, 0.4, 0.65, 1.25
Working Distance16mm (4X), 9.2mm (10X), 2.5mm (20X), 0.68mm (40X), 0.21mm (100X)
Field of View22mm
Field Number22
Parfocal Length45mm
Optical SystemFinite

Specifications are taken from the AMADA product data for this item. Confirm critical dimensions with us in writing before ordering.

Comparison With Other Options

Standard Plan Infinity Objective without Phase ContrastThe PH series adds phase contrast rings to enhance contrast in transparent specimens; use when imaging live cells or unstained tissues without staining.
Finite Series without Phase ContrastThe PH series includes phase contrast functionality, while the standard finite series does not; choose PH when contrast is needed for low-contrast samples.
Achromatic Phase Contrast ObjectivesPlan objectives offer improved flatness and reduced aberration across the field compared to achromatic designs, especially at higher magnifications.

Use Guide

  • Ensure the microscope is set to the correct optical system (infinite or finite) before mounting the objective
  • Use the 45mm parfocal length to maintain focus when switching between objectives
  • For 100XPH, use immersion oil and ensure the lens is clean to avoid air bubbles
  • Match the field number (25mm or 22mm) to the eyepiece and camera sensor size to avoid vignetting
  • Clean the objective lens with lens paper and alcohol after use, especially after oil immersion
Answers

Frequently Asked Questions

What is the difference between the infinite and finite series?

The infinite series has longer working distances and is designed for infinity-corrected microscopes; the finite series has shorter working distances and is for finite-corrected systems.

Can I use the 100XPH objective without oil?

No. The 100XPH objective requires immersion oil to achieve the full numerical aperture of 1.25 and proper resolution.

What is the parfocal length of these objectives?

All objectives have a 45mm parfocal length, so focus remains consistent when switching between them.

Are these objectives compatible with all microscopes?

They are compatible with microscopes that support 45mm parfocal length and the correct optical system (infinite or finite). Confirm with your microscope specifications.

What field of view do these objectives provide?

The infinite series provides a 25mm field of view; the finite series provides a 22mm field of view.

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