Parfocal Length 45mm Plan Infinite Biological Objective
The Parfocal Length 45mm Plan Infinite Biological Objective is a series of high-precision objective lenses designed for use with biological microscopes that employ an infinity optical system.
Product Overview
The Parfocal Length 45mm Plan Infinite Biological Objective is a series of high-precision objective lenses designed for use with biological microscopes that employ an infinity optical system. Each objective maintains a consistent parfocal length of 45mm, ensuring that focus remains stable when switching between magnifications. The series includes 4X, 10X, 20X, 40X, 60X, and 100X magnifications, with numerical apertures ranging from 0.1 to 1.25 and working distances from 0.22mm to 16.15mm. These objectives are suitable for routine biological specimen observation, cell counting, tissue analysis, and general laboratory work under brightfield illumination.
- ModelParfocal Length 45mm Plan Infinite Biological Objective
- Magnification4X, 10X, 20X, 40X, 60X, 100X
- Numerical Aperture0.1 (4X), 0.25 (10X), 0.45 (20X), 0.7 (40X), 0.7 (40X LWD), 0.85 (60X), 1.25 (100X)
- Working Distance16.15mm (4X), 9.57mm (10X), 2.8mm (20X), 0.55mm (40X), 1.8mm (40X LWD), 0.65mm (60X), 0.22mm (100X)
- Field of View25mm (FN) for all objectives
- Parfocal Length45mm
Key Benefits
- 45mm parfocal length ensures focus stability when switching between objectives
- Plan infinite optical system provides flat field imaging across the entire field of view
- Semiapochromat and high NA objectives improve resolution and contrast at 20X, 40X, and 60X
- Working distances from 0.22mm to 16.15mm allow for use with thick specimens and shallow depth of field applications
- Standard 25mm field of view (FN) ensures compatibility with most eyepieces and camera sensors
- 100X objective with 1.25 NA delivers high resolution for fine cellular detail under oil immersion
Application Scenarios
- Routine histology section examination with a standard biological microscope
- Cell counting and morphology assessment in a clinical or research lab
- Tissue culture monitoring under brightfield illumination
- Microbial colony analysis on agar plates with 4X and 10X objectives
- High-resolution observation of cellular structures using the 60X and 100X objectives with immersion oil
- Laboratory training sessions where consistent focus is required across multiple magnifications
Who It Is For
- Clinical and veterinary laboratories performing routine specimen analysis
- Research labs conducting cell biology and histology studies
- University teaching labs for biology and life sciences instruction
- Quality control labs in pharmaceutical and biotech industries
- Biological research technicians working with fixed or stained tissue samples
Problems It Solves
- Inconsistent focus when switching objectives due to varying parfocal lengths
- Curved or distorted field of view at higher magnifications with non-plan objectives
- Limited resolution and contrast in high-magnification observations without semiapochromat or high NA optics
- Incompatibility with standard eyepieces or cameras due to mismatched field of view
- Insufficient working distance for handling thick specimens or using tools under the objective
Technical Features
- Infinity optical system with plan correction for flat field imaging
- Parfocal length of 45mm across all magnifications for consistent focus
- Numerical aperture from 0.1 (4X) to 1.25 (100X) for varying resolution and light gathering
- Working distance ranges from 0.22mm (100X) to 16.15mm (4X), suitable for different specimen types
- Field of view (FN) standardized at 25mm across all objectives
- Semiapochromat correction at 20X, 40X, and 60X reduces chromatic aberration
- 100X objective designed for oil immersion with 1.25 NA and 0.22mm working distance
- LWD (Long Working Distance) 40X objective with 1.8mm working distance for thicker samples
Specifications
| Model | Parfocal Length 45mm Plan Infinite Biological Objective |
|---|---|
| Magnification | 4X, 10X, 20X, 40X, 60X, 100X |
| Numerical Aperture | 0.1 (4X), 0.25 (10X), 0.45 (20X), 0.7 (40X), 0.7 (40X LWD), 0.85 (60X), 1.25 (100X) |
| Working Distance | 16.15mm (4X), 9.57mm (10X), 2.8mm (20X), 0.55mm (40X), 1.8mm (40X LWD), 0.65mm (60X), 0.22mm (100X) |
| Field of View | 25mm (FN) for all objectives |
| Parfocal Length | 45mm |
| Optical System | Plan Infinite |
| Correction | Plan, Semiapochromat (20X, 40X, 60X) |
| Immersion Type | Dry (4X-60X), Oil (100X) |
| Mount Type | Standard 25mm objective thread |
Specifications are taken from the AMADA product data for this item. Confirm critical dimensions with us in writing before ordering.
Customization Options
- Available in standard 25mm thread mount for compatibility with most biological microscopes
- No options for alternative magnifications, NA, or working distances beyond the listed models
- No custom coatings or mounting configurations offered
Comparison With Other Options
| Standard finite biological objective | The plan infinite system provides a flat field across the entire view, while finite objectives show curvature at the edges, especially at higher magnifications. |
|---|---|
| Non-parfocal objective series | Switching between magnifications in this series maintains focus; non-parfocal objectives require refocusing after each change, slowing inspection workflows. |
| Low NA objectives (e.g., 0.1-0.25) | Higher NA objectives (0.45-1.25) offer greater resolution and light gathering, improving image clarity on fine cellular details and stained sections. |
Use Guide
- Ensure the microscope's tube length is set to infinity for proper focus with this objective series
- Use immersion oil only with the 100X objective; clean the lens immediately after use
- Start with the lowest magnification (4X) to locate the specimen before switching to higher powers
- Do not touch the front lens element; use a lens brush or air blower for cleaning
- Verify that the objective is fully seated in the nosepiece to maintain parfocal alignment
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View DetailsFrequently Asked Questions
What is the parfocal length of this objective series?
All objectives in this series have a parfocal length of 45mm, ensuring focus stability when switching between magnifications.
Can I use these objectives with a standard biological microscope?
Yes, provided the microscope uses an infinity optical system and has a 25mm objective thread mount.
Is the 100X objective designed for oil immersion?
Yes, the 100X objective is designed for oil immersion and requires immersion oil for proper operation.
What is the working distance for the 40X objective?
The standard 40X objective has a working distance of 0.55mm; the LWD version has 1.8mm for thicker specimens.
Do these objectives require special cleaning?
Yes, clean with lens paper and lens cleaning fluid. Avoid touching the front element. Use a blower for dust on the oil immersion ring.
Are these objectives compatible with digital cameras?
Yes, provided the camera is mounted on a standard 25mm tube and the microscope is infinity corrected.
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