195 Semi-plan Achromatic Objective Lens for Biological Microscope
The 195 Semi-plan Achromatic Objective Lens is a 40X magnification objective designed for biological microscopes with a 0.65 numerical aperture and a 4.5mm working distance.
Product Overview
The 195 Semi-plan Achromatic Objective Lens is a 40X magnification objective designed for biological microscopes with a 0.65 numerical aperture and a 4.5mm working distance. It provides corrected chromatic and spherical aberration across the central field, suitable for routine staining and brightfield microscopy in teaching, clinical and research labs. The lens is supplied with a 23mm diameter barrel and a 160mm tube length specification.
- Optical DesignSemi-plan Achromatic
- CorrectionChromatic and spherical aberration in central field
- Mount TypeStandard nosepiece
- Illumination TypeBrightfield
- Use CaseStained and transparent biological specimens
Key Benefits
- 40X magnification with 0.65 NA delivers clear detail for cell and tissue structures
- Semi-plan correction reduces field curvature and chromatic aberration in the central 18mm diameter
- 4.5mm working distance allows access to thick specimens and coverslips without contact
- 23mm barrel diameter fits standard nosepiece mounts on biological microscopes
- 160mm tube length ensures compatibility with standard optical paths in biological microscopes
Application Scenarios
- Routine histology slide inspection in a clinical pathology lab
- Teaching cell structure and tissue layers in a university biology class
- Microbial colony analysis in a research lab using stained samples
- Quality control of cultured cells in a biotech production environment
- Documenting specimen features under brightfield illumination with a camera
Who It Is For
- Teaching laboratories in secondary schools and universities
- Clinical and veterinary pathology labs performing routine staining
- Research labs conducting basic biological microscopy
- Biotechnology and pharmaceutical quality control departments
- Microscopy technicians maintaining standard biological inspection workflows
Problems It Solves
- A standard achromatic objective with no field correction causes blurred edges in the image at higher magnifications
- Insufficient working distance forces the lens to touch the specimen, risking damage to both lens and slide
- Mismatched barrel diameter prevents mounting on standard microscope nosepieces
- Lack of tube length specification leads to focus issues when used on different microscopes
- No numerical aperture specification makes it hard to compare resolution performance
Technical Features
- Semi-plan achromatic optical design with correction for chromatic and spherical aberration
- 40X magnification with 0.65 numerical aperture for improved resolution and depth of field
- 4.5mm working distance allows safe focusing on thick or uneven specimens
- 23mm barrel diameter fits standard nosepiece mounts on biological microscopes
- 160mm tube length ensures correct optical path length in standard biological microscopes
- Designed for brightfield illumination with stained or transparent specimens
Specifications
| Optical Design | Semi-plan Achromatic |
|---|---|
| Correction | Chromatic and spherical aberration in central field |
| Mount Type | Standard nosepiece |
| Illumination Type | Brightfield |
| Use Case | Stained and transparent biological specimens |
Specifications are taken from the AMADA product data for this item. Confirm critical dimensions with us in writing before ordering.
Customization Options
- Available in 40X magnification only as specified
- No options for different numerical apertures or working distances
- No interchangeable mounting threads or barrel sizes offered
- No coating variations or special surface treatments available
Comparison With Other Options
| Standard achromatic objective without semi-plan correction | The 195 lens reduces field curvature and color fringing in the central 18mm area, improving image clarity across the field compared to uncorrected objectives. |
|---|---|
| 40X objective with 0.75 NA | The 0.65 NA in the 195 lens provides a longer working distance and shallower depth of field, better suited for thick specimens and routine observation. |
| 40X objective with 100mm tube length | The 160mm tube length ensures compatibility with standard biological microscopes; 100mm systems require different optical design and are not interchangeable. |
Use Guide
- Ensure the microscope tube length is set to 160mm before mounting the lens
- Use a clean, dry lens cloth to wipe the front element before and after use
- Avoid touching the optical surfaces with fingers to prevent oil residue
- Do not force the lens into the nosepiece; it should seat smoothly with a click
- Check the working distance when focusing on thick specimens to prevent contact
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View DetailsFrequently Asked Questions
What is the working distance of this objective?
4.5mm. This allows safe focusing on thick specimens without contact with the slide.
Is this objective compatible with my biological microscope?
Yes, if your microscope has a 160mm tube length and a standard nosepiece with 23mm barrel fit.
Can I use this lens for oil immersion?
No. This is a dry objective designed for air immersion only. Oil immersion requires a different objective with a higher NA and oil immersion design.
What magnification does this lens provide?
40X. The total magnification depends on the eyepiece used, typically 10X, resulting in 400X total magnification.
Does this lens correct for chromatic aberration?
Yes. It is a semi-plan achromatic lens, correcting for chromatic and spherical aberration in the central field.
Can I use this lens with a digital camera?
Yes. It is compatible with standard C-mount or photo tube adapters if the camera is mounted on the microscope.
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