AMADA INSTRUMENT CO.,LIMITED — Nanjing China
Inverted Biological Microscope

XIB300 Inverted Lab Biological Microscope with LED Illumination

The XIB300 is an inverted biological microscope with a long working distance plan achromatic objective system and a 45° inclined eyepiece tube.

Product Overview

The XIB300 is an inverted biological microscope with a long working distance plan achromatic objective system and a 45° inclined eyepiece tube. It supports phase contrast imaging with PHP2 objectives and includes a fixed stage with mechanical movement and multiple culture dish holders. The microscope uses a 6V30W halogen illumination system with brightness control and frosted glass and color filters. It is designed for observing live cells, cultures, and specimens in petri dishes or culture vessels on a bench.

  • Optical systemInfinity
  • Objective typePlan achromatic
  • Objective magnification10X, 20X, 40X
  • Objective NA0.25, 0.40, 0.60
  • Working distance4.3mm (10X), 8.0mm (20X), 3.5mm (40X)
  • Cover glass thickness1.2mm

Key Benefits

  • Long working distance objectives (4.3mm to 8.0mm) allow observation of thick samples and culture vessels without interference
  • Phase contrast objectives (PHP2) enable high-contrast imaging of transparent or unstained biological specimens
  • Mechanical stage with 77mm longitudinal and 114mm transverse movement supports precise positioning of large samples
  • Multiple culture dish holders (86x129.5mm, 34x77.5mm, 57x82mm) accommodate different dish sizes and types
  • Coaxial coarse and fine focus with 2μm minimum division allows precise depth control for thin sectioning and focusing
  • Inclined 45° eyepiece tube with 53~75mm interpupillary adjustment improves operator comfort during extended use
  • Built-in condenser with 55mm working distance and turnplate phase contrast unit supports phase contrast imaging without moving the stage

Application Scenarios

  • Observation of live cell cultures in petri dishes or culture flasks on a laboratory bench
  • Phase contrast imaging of unstained or transparent biological samples such as bacteria, yeast, and protozoa
  • Quality control of cell cultures in biotech or pharmaceutical labs using phase contrast to visualize cell morphology
  • Teaching laboratory sessions where students observe live specimens with minimal preparation
  • Microscopic inspection of culture vessels and growth patterns in microbiology and cell biology research

Who It Is For

  • Biological and cell biology research laboratories
  • Clinical and veterinary diagnostic labs performing cell analysis
  • Teaching and training labs in universities and technical schools
  • Pharmaceutical and biotech quality control departments
  • Microbiology labs conducting live culture monitoring

Problems It Solves

  • Standard upright microscopes cannot accommodate culture dishes or petri dishes with sufficient clearance
  • Short working distance objectives require removal of the culture vessel or risk damaging the objective
  • Lack of phase contrast capability limits visibility of transparent or unstained specimens
  • Fixed stages prevent efficient scanning of large or irregularly shaped samples
  • Inadequate focus precision makes it difficult to track depth changes in thick or layered samples

Technical Features

  • Infinity optical system with long working distance plan achromatic objectives
  • Phase contrast objectives (PHP2) with 10X, 20X, and 40X magnifications and matching working distances
  • Coaxial coarse and fine focus with adjustable tension and 2μm minimum division for precise focusing
  • Quintuple nosepiece for quick objective switching
  • Fixed stage with mechanical moving device (77mm x 114mm travel range)
  • Inclined 45° eyepiece tube with 53~75mm interpupillary adjustment
  • Long working distance condenser with 55mm clearance and turnplate phase contrast unit
  • 6V30W halogen illumination with brightness control and frosted glass and color filters
  • Multiple culture dish holders for different dish sizes and shapes

Specifications

Optical systemInfinity
Objective typePlan achromatic
Objective magnification10X, 20X, 40X
Objective NA0.25, 0.40, 0.60
Working distance4.3mm (10X), 8.0mm (20X), 3.5mm (40X)
Cover glass thickness1.2mm
Phase contrast objectivePHP2
Eyepiece tube angle45°
Interpupillary adjustment53~75mm
Focus systemCoaxial coarse and fine focus
Fine focus division2μm
NosepieceQuintuple
Stage typeFixed stage
Stage size227mm x 208mm (overall), Ф118mm (rotundity)
Mechanical stage travel77mm (longitudinal) x 114mm (transverse)
Condenser working distance55mm
Condenser typeLong working distance with turnplate phase contrast unit
Illumination system6V30W halogen
Brightness controlYes
FiltersFrosted glass, blue, green
EyepieceWF10X (field number: Φ22mm)
Optional eyepieceDividing eyepiece (Φ22mm, 0.10mm/div)
Optional phase contrast objectivePL L20X/0.40 PHP2, PL L40X/0.60 PHP2
Optional camera adapter0.5X, 1X
Optional camera outputDV-1 (380/520 TV line, USB 0.42M pixel), DV-2 (1.3M, 2.0M, 3.0M pixel USB), DV-3 (380/520 TV line video)

Specifications are taken from the AMADA product data for this item. Confirm critical dimensions with us in writing before ordering.

Customization Options

  • Option to select binocular or trinocular head configuration
  • Add phase contrast objectives (PHP2) for transparent specimen imaging
  • Upgrade to higher-resolution camera adapters (DV-2 with 1.3M, 2.0M, or 3.0M pixel USB output)
  • Attach optional dividing eyepiece for measurement calibration
  • Choose between different culture dish holders based on sample size

Comparison With Other Options

Standard upright biological microscopeThe inverted design allows direct observation of culture dishes and petri dishes without removing them from the stage, and the long working distance objectives prevent contact with the sample.
Microscope without phase contrastThe XIB300 includes PHP2 phase contrast objectives, enabling clear imaging of transparent or unstained biological specimens that would be difficult to see under brightfield illumination.
Microscope with fixed focusThe XIB300 has a coaxial coarse and fine focus with 2μm division, allowing precise depth control for layered or thick samples, unlike fixed focus systems that lack adjustment precision.

Use Guide

  • Adjust the interpupillary distance between 53mm and 75mm before focusing to ensure a single merged image
  • Start focusing at the highest magnification objective, then move to lower magnifications to maintain focus across the range
  • Use the mechanical stage to position large samples; the 77mm x 114mm travel range supports full coverage of standard culture dishes
  • Select the appropriate culture dish holder based on the dish size and secure it in place before observation
  • For phase contrast imaging, ensure the turnplate phase contrast unit is aligned with the objective and the condenser is set to phase mode
Answers

Frequently Asked Questions

What is the working distance for the 10X objective?

4.3mm. The 20X objective has 8.0mm, and the 40X has 3.5mm. All are designed for 1.2mm cover glass thickness.

Can I observe live cell cultures in petri dishes?

Yes. The long working distance objectives and inverted design allow direct observation of culture vessels without removing them from the stage.

Does the microscope support phase contrast imaging?

Yes. It includes PHP2 phase contrast objectives for 10X, 20X, and 40X magnifications, and a turnplate phase contrast unit in the condenser.

What camera options are available?

Available camera adapters include DV-1 (USB 0.42M pixel), DV-2 (1.3M, 2.0M, 3.0M pixel USB), and DV-3 (380/520 TV line video output).

Can I use this microscope with a digital camera?

Yes. The microscope supports camera adapters (0.5X, 1X) and has USB and video output options for digital capture.

What stage movement range is available?

The mechanical stage moves 77mm longitudinally and 114mm transversely, covering the full area of standard culture dishes.

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