XIB500 Inverted Lab Biological Microscope for Clinical Diagnostics
The XIB500 is an inverted biological microscope with a Seidentopf trinocular head, Koehler illumination, and a 4-hole rotating disc phase contrast condenser.

Product Overview
The XIB500 is an inverted biological microscope with a Seidentopf trinocular head, Koehler illumination, and a 4-hole rotating disc phase contrast condenser. It supports 4X to 40X infinity plan objectives with working distances from 3.4mm to 12.1mm, and includes a 12V/50W halogen lamp with auto power on-off. The microscope is designed for clinical diagnostics, cell culture observation, and laboratory-based biological sample inspection with full compatibility for digital photography via C-mount adaptor.
- Optical systemInfinity Plan
- Head typeSeidentopf Trinocular, 45° inclined
- Interpupillary adjustment48-76mm
- EyepieceWF10X/22mm (adjustable)
- Objective lensesLPL 4X/0.11 W.D.=12.1mm, LPL 10X/0.25 W.D.=8.3mm, LPL 20X/0.40 W.D.=7.2mm, LPL 40X/0.60 W.D.=3.4mm
- Phase contrast objectivesLPL Ph P 4X/0.10 W.D.=9.2mm, LPL Ph P 10X/0.25 W.D.=8.3mm, LPL Ph P 20X/0.40 W.D.=7.2mm, LPL Ph P 40X/0.60 W.D.=3.4mm
Key Benefits
- Inverted design allows direct observation of culture dishes, slides, and 96-well plates without flipping the sample
- Seidentopf trinocular head at 45° inclination with 48-76mm interpupillary adjustment supports long-term viewing and camera integration
- Koehler illumination with 12V/50W halogen lamp ensures even, bright lighting across all magnifications
- Phase contrast objectives (4X, 10X, 20X, 40X) enhance contrast in transparent biological specimens
- C-mount adaptor provides 100% light transmission to SLR digital cameras for high-resolution documentation
- Auto power on-off system reduces energy use and prevents lamp burnout from prolonged idle time
- Mechanical stage with 128x80mm movement range and 96-well plate compatibility supports high-throughput sample handling
- Working distances from 3.4mm to 12.1mm accommodate various sample thicknesses and dish sizes
Application Scenarios
- Clinical diagnostics in hospital or research labs using blood smears, tissue sections, and cell cultures
- Observation of live cell cultures in 65mm, 54mm, or 35mm culture dishes with minimal disturbance
- High-throughput screening of 96-well plates in microbiology or drug testing labs
- Digital documentation of cell morphology using a SLR camera via C-mount adaptor
- Phase contrast imaging of unstained cells and microorganisms in microbiology labs
- Long-duration observation sessions with reduced eye strain due to the 45° inclined trinocular head
Who It Is For
- Clinical laboratories performing blood cell counts and tissue analysis
- Research labs studying live cell dynamics and cell culture growth
- University teaching labs demonstrating phase contrast and inverted microscopy
- Pharmaceutical and biotech labs conducting cell-based assays
- Microbiology labs analyzing bacterial colonies and microbial cultures
Problems It Solves
- Standard upright microscopes require flipping samples to observe from below; the inverted design eliminates this step
- Lack of phase contrast in low-cost microscopes makes transparent samples hard to see; the XIB500 includes phase contrast objectives
- Manual focus and lighting adjustment in older models lead to inconsistent results; Koehler illumination ensures uniform lighting
- Separate camera and microscope setups often mismatch optical paths; the C-mount adaptor ensures full light transfer
- No auto power management leads to lamp degradation; the auto on-off system extends lamp life
- Small mechanical stages limit sample movement; the XIB500 stage supports 96-well plates and large slide sizes
Technical Features
- Inverted optical design with infinity plan objectives for high-resolution imaging
- Seidentopf trinocular head at 45° with 48-76mm interpupillary adjustment for ergonomic viewing
- Koehler illumination with 12V/50W halogen lamp and 100V-240V input for global use
- 4-hole rotating disc phase contrast condenser with N.A. 0.4 and 45mm working distance
- Quintuple nosepiece for quick objective switching
- Mechanical stage with 210x241mm size, φ110mm round slide support, and 128x80mm movement range
- 96-well plate holder compatible with standard lab formats
- C-mount adaptor with 1X, 0.5X, 0.75X options and focus adjustment for digital photography
- Auto power on-off system activates upon user approach and deactivates after 10 minutes of inactivity
- Blue, green, and amber/grey filters for contrast enhancement in different sample types
Specifications
| Optical system | Infinity Plan |
|---|---|
| Head type | Seidentopf Trinocular, 45° inclined |
| Interpupillary adjustment | 48-76mm |
| Eyepiece | WF10X/22mm (adjustable) |
| Objective lenses | LPL 4X/0.11 W.D.=12.1mm, LPL 10X/0.25 W.D.=8.3mm, LPL 20X/0.40 W.D.=7.2mm, LPL 40X/0.60 W.D.=3.4mm |
| Phase contrast objectives | LPL Ph P 4X/0.10 W.D.=9.2mm, LPL Ph P 10X/0.25 W.D.=8.3mm, LPL Ph P 20X/0.40 W.D.=7.2mm, LPL Ph P 40X/0.60 W.D.=3.4mm |
| Annular spot | 10X, 20X, 40X |
| Nosepiece | Quintuple |
| Mechanical stage | 210x241mm, φ110mm round slide, 128x80mm movement range |
| Culture dish holder | 65mm, 54mm, 35mm |
| Condenser | 4-hole rotating disc phase contrast, N.A. 0.4, W.D. 45mm, up-down adjustable |
| Illumination | 12V/50W halogen, 100V-240V input |
| LED option | 5WLED, 100V-240V input |
| Auto power on-off | Power off after 10 minutes idle, power on on approach |
| Filters | Blue, Green, Amber/Grey |
| C-mount adaptor | 1X, 0.5X, 0.75X, focus adjustable |
| Epi fluorescence | 100W mercury lamp, 5WLED, B/G/V/UV filters available |
| Packing size | 660mmx590mmx325mm |
| Gross weight | 18 kgs |
| Net weight | 13.5 kgs |
Specifications are taken from the AMADA product data for this item. Confirm critical dimensions with us in writing before ordering.
Customization Options
- Choose between standard halogen lamp or optional 5WLED illumination
- Add phase contrast objectives (4X, 10X, 20X, 40X) as needed
- Select culture dish holders (65mm, 54mm, 35mm) based on sample type
- Upgrade to epi fluorescence illumination with mercury lamp and filter set
- Add C-mount adaptor with 1X, 0.5X, or 0.75X magnification options
- Request custom mechanical stage configuration for specific plate formats
Comparison With Other Options
| Standard upright biological microscope | The inverted design allows direct observation of samples in culture dishes and plates without flipping, reducing handling and contamination risk. |
|---|---|
| Microscope without phase contrast | The XIB500 includes phase contrast objectives for enhanced visibility of transparent, unstained biological specimens. |
| Microscope without auto power control | The auto on-off system prevents lamp burnout and reduces energy use during idle periods. |
Use Guide
- Adjust the interpupillary distance between 48mm and 76mm before use to ensure a single merged image
- Set the condenser height and center it using the centering telescope for optimal Koehler illumination
- Use the 12V/50W halogen lamp for high brightness; switch to 5WLED for lower heat and energy use
- For digital photography, attach the C-mount adaptor and select the correct magnification (1X, 0.5X, 0.75X) based on sensor size
- When using phase contrast, ensure the annular diaphragm matches the objective's phase ring
- Place culture dishes on the holder and center them before focusing to avoid edge distortion
- Allow the lamp to warm up for 2-3 minutes before starting long observation sessions
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Frequently Asked Questions
What is the working distance for the 40X objective?
3.4mm. The 40X objective has a working distance of 3.4mm, suitable for thin samples and high magnification.
Can I use this microscope with a digital SLR camera?
Yes. The C-mount adaptor provides 100% light transmission to SLR cameras from Canon or Nikon.
Does the microscope support phase contrast imaging?
Yes. It includes phase contrast objectives (4X, 10X, 20X, 40X) and a rotating disc condenser with annular rings.
Is the lamp auto-on/off system reliable?
Yes. The system detects user presence and powers off after 10 minutes of inactivity, then powers on when approached.
Can I observe live cell cultures in 96-well plates?
Yes. The mechanical stage supports 96-well plates, and the inverted design allows direct observation without moving the plate.
What is the maximum magnification with the 10X eyepiece?
With the 40X objective and 10X eyepiece, the total magnification is 400X. Higher magnification is possible with optional objectives and eyepieces.
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