XIB400 Inverted Laboratory Biological Microscope for Research

Key Benefits

• Inverted design allows observation of culture dishes, slides, and plates without interference from the stage
• Infinity plan objectives deliver flat, high-contrast images across the field at all magnifications
• Phase contrast objectives (10X, 20X, 40X) enhance contrast in transparent specimens like live cells
• Epi fluorescence with mercury lamp and filter selection enables detection of labeled proteins and nucleic acids
• Trinocular head with 100:0 or 80:20 light split allows simultaneous viewing and digital capture
• Koehler illumination ensures even, glare-free lighting across the field of view
• Quickly detachable condenser with 72mm working distance simplifies setup and cleaning
• C-mount adapters and digital camera compatibility support image capture and documentation

Application Scenarios

• Live cell imaging in tissue culture labs using phase contrast and epi fluorescence
• Observation of microorganisms in liquid cultures in petri dishes or flasks
• Inspection of cell monolayers and tissue sections on glass slides
• Documentation of fluorescently labeled samples for research reports and publications
• Teaching histology and cell biology in university laboratories with real-time image capture
• Quality control in biotech labs for cell viability and morphology checks
• Long-term observation of growing cultures with stable illumination and focus

Who It Is For

• Research laboratories in universities and biotech firms
• Clinical and veterinary diagnostic labs using cell cultures
• Teaching labs in biology, microbiology, and medical education
• Biopharmaceutical quality control and process development teams
• Cell culture and tissue engineering labs
• Microbiology and environmental testing labs

Problems It Solves

• Traditional upright microscopes cannot accommodate large culture dishes or plates
• Low contrast in transparent specimens like live cells is difficult to resolve without phase contrast
• Separate fluorescence and transmitted light setups require multiple instruments
• Manual illumination adjustment leads to uneven lighting and glare
• Lack of digital capture capability forces the operator to describe images instead of recording them
• Fixed light distribution in trinocular heads limits simultaneous viewing and recording
• Inconsistent focus and image quality across magnifications due to non-infinity optics

Technical Features

• Seidentopf trinocular head with 45° inclination and 48-76mm interpupillary adjustment
• Quintuple nosepiece for five objectives in one rotation
• Infinity optical system with plan objectives for flat, distortion-free images
• Koehler illumination with 6V/30W lamp and 100V~240V input voltage
• Quickly detachable condenser with 72mm working distance (with condenser), 195mm (without)
• Epi fluorescence unit with field diaphragm and center adjustment
• 100W mercury lamp (OSRAM) with digital power box for stable operation
• Selectable fluorescence filters: B, G, V, UV for different fluorophores
• C-mount 1x and 0.5x adapters for digital camera integration
• Mechanical stage with 210x241mm size and 128x80mm moving range for 96-well plates
• Culture dish holders for 65mm, 54mm, and 35mm dishes

Specifications

Specifications are taken from the AMADA product data for this item. Confirm critical dimensions with us in writing before ordering.

Customization Options

• Choose between WF10x/22mm and WF10x/23mm eyepieces
• Select phase contrast objectives for 10X, 20X, or 40X
• Add annular spot objectives for 10X, 20X, or 40X
• Order with or without epi fluorescence unit and mercury lamp
• Select fluorescence filters: B, G, V, UV
• Choose C-mount 1x or 0.5x adapter for digital camera
• Add culture dish holders for 65mm, 54mm, or 35mm dishes
• Request mechanical stage with 96-well plate holder

Comparison With Other Options

Use Guide

• Adjust the interpupillary distance to 48-76mm before focusing to ensure a single merged image
• Use Koehler illumination to align the lamp and condenser for even lighting
• For fluorescence, ensure the mercury lamp is warmed up and the correct filter is in place
• Use the 0.5x C-mount adapter if your camera requires focus adjustment
• Place the culture dish on the stage with the bottom facing up for optimal focus
• Use the 96-well plate holder for high-throughput screening and consistent positioning
• Detach the condenser when observing thick samples or large dishes to avoid interference