AMADA INSTRUMENT CO.,LIMITED — Nanjing China
USB3.0 CCD Microscope Camera

FL-20BW Mono Cooled SONY CMOS Camera for Fluorescence Imaging

The FL-20BW is a mono cooled CMOS camera with a 20MP SONY IMX183CLK-J sensor, designed for fluorescence imaging on microscopes.

Product Overview

The FL-20BW is a mono cooled CMOS camera with a 20MP SONY IMX183CLK-J sensor, designed for fluorescence imaging on microscopes. It delivers 20 megapixels at 5472 x 3648 resolution with a 2.4μm pixel size, and achieves a dark current of 0.001e-/pixel/s through active cooling. The camera connects via USB3.0 and supports up to four units simultaneously in SDK mode, making it suitable for multi-camera setups in research and inspection environments.

  • Sensor TypeCMOS
  • Sensor ModelSONY IMX183CLK-J
  • Color/MonoMono
  • Array Diagonal15.86mm
  • Resolution20MP, 5472(H) x 3648(V)
  • Pixel Size2.4μm x 2.4μm

Key Benefits

  • 20 megapixel resolution at 5472 x 3648 captures high-detail fluorescence images in a single exposure
  • Active cooling reduces dark current to 0.001e-/pixel/s, minimizing hot pixels during long exposures
  • 2.4μm pixel size balances resolution and sensitivity for low-light fluorescence applications
  • USB3.0 interface enables 8 frames per second at full resolution with low readout noise of 0.6e
  • Supports up to four cameras simultaneously via SDK, enabling synchronized imaging in multi-channel setups

Application Scenarios

  • Long exposure fluorescence imaging in biological or material science labs where dark current must be minimized
  • Multi-camera imaging setups for time-lapse or spectral channel capture in confocal or epifluorescence microscopy
  • High-resolution documentation of fluorescent samples in clinical or veterinary pathology
  • Research applications requiring low readout noise and high dynamic range in low-light conditions
  • Automated inspection lines using fluorescence to detect defects in semiconductor or micro-optical components

Who It Is For

  • Research laboratories conducting fluorescence microscopy in biology, materials science, or medical diagnostics
  • Clinical and veterinary pathology labs requiring high-resolution, low-noise image capture
  • Semiconductor and micro-optics inspection teams using fluorescence to detect surface defects
  • Academic institutions teaching advanced microscopy techniques with digital imaging
  • Industrial quality control teams using fluorescence for non-destructive defect detection

Problems It Solves

  • High dark current in uncooled CMOS cameras causes false signals during long exposures in fluorescence imaging
  • Low-resolution cameras require multiple captures and stitching, increasing processing time and risk of misalignment
  • Lack of multi-camera support limits synchronized imaging across multiple channels or microscopes
  • Separate power supplies and data cables increase setup complexity and cabling clutter
  • Inadequate cooling leads to thermal noise that degrades image quality in extended imaging sessions

Technical Features

  • Sony IMX183CLK-J CMOS sensor with 15.86mm diagonal and 20MP resolution at 5472 x 3648
  • Active cooling system reduces dark current to 0.001e-/pixel/s
  • 2.4μm x 2.4μm pixel size with 16ke- full-well capacity for high dynamic range
  • 8 frames per second at full resolution over USB3.0 interface
  • Rolling shutter mode with exposure time from 16μs to 60 minutes
  • Supports binning modes: 2x2, 3x3, 4x4, 8x8 for faster frame rates or lower noise
  • 16-bit or 8-bit bit depth output for high dynamic range or file size control
  • Standard C-mount optical interface for compatibility with microscope objectives
  • SDK supports up to four cameras simultaneously for synchronized capture

Specifications

Sensor TypeCMOS
Sensor ModelSONY IMX183CLK-J
Color/MonoMono
Array Diagonal15.86mm
Resolution20MP, 5472(H) x 3648(V)
Pixel Size2.4μm x 2.4μm
Effective Area13.1mm x 8.8mm
Full-Well Capacity16ke
Frame Rate8fps @ USB3.0
Peak QE84% @ 495nm
Dark Current0.001e-/p/s
Readout Noise0.6e
DSNU0.2e
PRNU0.8%
Shutter ModeRolling
Exposure Time16μs - 60min
Binning2x2, 3x3, 4x4, 8x8
PC SoftwareMosaic V2
Picture FormatTIFF/JPG/PNG/DICOM
Data InterfaceUSB3.0
Multiple CamerasSupports 4 cameras simultaneously in SDK
SDKC/C++, C#, Directshow/Twain
Optical InterfaceStandard C Mount
Bit Depth16/8bit
Power12V
Operating Temperature0 degC - 40 degC
Humidity10% - 85%
Dimensions85mm x 85mm x 112mm
Camera Weight980g
Operating SystemWindows 7/10 (32 Bit/64 Bit)/Mac
PC ConfigurationCPU: Intel Core i5 or better (Quad or more Core), RAM: 8G or more

Specifications are taken from the AMADA product data for this item. Confirm critical dimensions with us in writing before ordering.

Customization Options

  • Available with or without cooling module depending on application needs
  • Supports custom firmware configuration via SDK for integration into automated systems
  • Can be paired with optional C-mount adapters for non-standard objective flanges
  • Configurable bit depth and output format via software for specific data handling workflows

Comparison With Other Options

Uncooled CMOS fluorescence cameraThe FL-20BW's active cooling reduces dark current to 0.001e-/pixel/s, eliminating thermal noise during long exposures; uncooled models show increasing hot pixels over time
CCD fluorescence cameraThe FL-20BW matches CCD-level dark current performance at lower cost and with faster readout speeds and lower power consumption
Standard USB2.0 CMOS cameraThe FL-20BW uses USB3.0 to deliver 8fps at full resolution; USB2.0 cameras are limited to lower frame rates or reduced resolution

Use Guide

  • Ensure the microscope's optical path is aligned to the C-mount before mounting the camera
  • Use a 12V power supply with stable output to maintain consistent cooling performance
  • Set the exposure time to 16μs for fast events or up to 60 minutes for low-light fluorescence capture
  • Enable binning (2x2 or higher) to increase frame rate or reduce noise in low-signal conditions
  • Use the Mosaic V2 software to stitch multiple images or manage multi-camera synchronization
Answers

Frequently Asked Questions

What is the dark current level of the FL-20BW?

0.001e-/pixel/s, achieved through active cooling, which minimizes thermal noise during long exposures

Does the camera require external cooling?

Yes. The camera includes an active cooling system that maintains low dark current; it must be powered and connected via USB3.0 to operate

Can I use this camera with a microscope that has a different optical interface?

Yes, with a C-mount adapter. The standard interface is C-mount; custom adapters are available on request

How many cameras can run simultaneously?

The SDK supports up to four FL-20BW cameras at once for synchronized imaging across multiple channels

What operating systems are supported?

Windows 7/10 (32 Bit/64 Bit) and Mac. Confirm compatibility with the latest SDK release before ordering

What is the maximum frame rate at full resolution?

8 frames per second at 5472 x 3648 resolution over USB3.0

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