FL-20BW Mono Cooled SONY CMOS Camera for Fluorescence Imaging
The FL-20BW is a mono cooled CMOS camera with a 20MP SONY IMX183CLK-J sensor, designed for fluorescence imaging on microscopes.

Product Overview
The FL-20BW is a mono cooled CMOS camera with a 20MP SONY IMX183CLK-J sensor, designed for fluorescence imaging on microscopes. It delivers 20 megapixels at 5472 x 3648 resolution with a 2.4μm pixel size, and achieves a dark current of 0.001e-/pixel/s through active cooling. The camera connects via USB3.0 and supports up to four units simultaneously in SDK mode, making it suitable for multi-camera setups in research and inspection environments.
- Sensor TypeCMOS
- Sensor ModelSONY IMX183CLK-J
- Color/MonoMono
- Array Diagonal15.86mm
- Resolution20MP, 5472(H) x 3648(V)
- Pixel Size2.4μm x 2.4μm
Key Benefits
- 20 megapixel resolution at 5472 x 3648 captures high-detail fluorescence images in a single exposure
- Active cooling reduces dark current to 0.001e-/pixel/s, minimizing hot pixels during long exposures
- 2.4μm pixel size balances resolution and sensitivity for low-light fluorescence applications
- USB3.0 interface enables 8 frames per second at full resolution with low readout noise of 0.6e
- Supports up to four cameras simultaneously via SDK, enabling synchronized imaging in multi-channel setups
Application Scenarios
- Long exposure fluorescence imaging in biological or material science labs where dark current must be minimized
- Multi-camera imaging setups for time-lapse or spectral channel capture in confocal or epifluorescence microscopy
- High-resolution documentation of fluorescent samples in clinical or veterinary pathology
- Research applications requiring low readout noise and high dynamic range in low-light conditions
- Automated inspection lines using fluorescence to detect defects in semiconductor or micro-optical components
Who It Is For
- Research laboratories conducting fluorescence microscopy in biology, materials science, or medical diagnostics
- Clinical and veterinary pathology labs requiring high-resolution, low-noise image capture
- Semiconductor and micro-optics inspection teams using fluorescence to detect surface defects
- Academic institutions teaching advanced microscopy techniques with digital imaging
- Industrial quality control teams using fluorescence for non-destructive defect detection
Problems It Solves
- High dark current in uncooled CMOS cameras causes false signals during long exposures in fluorescence imaging
- Low-resolution cameras require multiple captures and stitching, increasing processing time and risk of misalignment
- Lack of multi-camera support limits synchronized imaging across multiple channels or microscopes
- Separate power supplies and data cables increase setup complexity and cabling clutter
- Inadequate cooling leads to thermal noise that degrades image quality in extended imaging sessions
Technical Features
- Sony IMX183CLK-J CMOS sensor with 15.86mm diagonal and 20MP resolution at 5472 x 3648
- Active cooling system reduces dark current to 0.001e-/pixel/s
- 2.4μm x 2.4μm pixel size with 16ke- full-well capacity for high dynamic range
- 8 frames per second at full resolution over USB3.0 interface
- Rolling shutter mode with exposure time from 16μs to 60 minutes
- Supports binning modes: 2x2, 3x3, 4x4, 8x8 for faster frame rates or lower noise
- 16-bit or 8-bit bit depth output for high dynamic range or file size control
- Standard C-mount optical interface for compatibility with microscope objectives
- SDK supports up to four cameras simultaneously for synchronized capture
Specifications
| Sensor Type | CMOS |
|---|---|
| Sensor Model | SONY IMX183CLK-J |
| Color/Mono | Mono |
| Array Diagonal | 15.86mm |
| Resolution | 20MP, 5472(H) x 3648(V) |
| Pixel Size | 2.4μm x 2.4μm |
| Effective Area | 13.1mm x 8.8mm |
| Full-Well Capacity | 16ke |
| Frame Rate | 8fps @ USB3.0 |
| Peak QE | 84% @ 495nm |
| Dark Current | 0.001e-/p/s |
| Readout Noise | 0.6e |
| DSNU | 0.2e |
| PRNU | 0.8% |
| Shutter Mode | Rolling |
| Exposure Time | 16μs - 60min |
| Binning | 2x2, 3x3, 4x4, 8x8 |
| PC Software | Mosaic V2 |
| Picture Format | TIFF/JPG/PNG/DICOM |
| Data Interface | USB3.0 |
| Multiple Cameras | Supports 4 cameras simultaneously in SDK |
| SDK | C/C++, C#, Directshow/Twain |
| Optical Interface | Standard C Mount |
| Bit Depth | 16/8bit |
| Power | 12V |
| Operating Temperature | 0 degC - 40 degC |
| Humidity | 10% - 85% |
| Dimensions | 85mm x 85mm x 112mm |
| Camera Weight | 980g |
| Operating System | Windows 7/10 (32 Bit/64 Bit)/Mac |
| PC Configuration | CPU: Intel Core i5 or better (Quad or more Core), RAM: 8G or more |
Specifications are taken from the AMADA product data for this item. Confirm critical dimensions with us in writing before ordering.
Customization Options
- Available with or without cooling module depending on application needs
- Supports custom firmware configuration via SDK for integration into automated systems
- Can be paired with optional C-mount adapters for non-standard objective flanges
- Configurable bit depth and output format via software for specific data handling workflows
Comparison With Other Options
| Uncooled CMOS fluorescence camera | The FL-20BW's active cooling reduces dark current to 0.001e-/pixel/s, eliminating thermal noise during long exposures; uncooled models show increasing hot pixels over time |
|---|---|
| CCD fluorescence camera | The FL-20BW matches CCD-level dark current performance at lower cost and with faster readout speeds and lower power consumption |
| Standard USB2.0 CMOS camera | The FL-20BW uses USB3.0 to deliver 8fps at full resolution; USB2.0 cameras are limited to lower frame rates or reduced resolution |
Use Guide
- Ensure the microscope's optical path is aligned to the C-mount before mounting the camera
- Use a 12V power supply with stable output to maintain consistent cooling performance
- Set the exposure time to 16μs for fast events or up to 60 minutes for low-light fluorescence capture
- Enable binning (2x2 or higher) to increase frame rate or reduce noise in low-signal conditions
- Use the Mosaic V2 software to stitch multiple images or manage multi-camera synchronization
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View DetailsFrequently Asked Questions
What is the dark current level of the FL-20BW?
0.001e-/pixel/s, achieved through active cooling, which minimizes thermal noise during long exposures
Does the camera require external cooling?
Yes. The camera includes an active cooling system that maintains low dark current; it must be powered and connected via USB3.0 to operate
Can I use this camera with a microscope that has a different optical interface?
Yes, with a C-mount adapter. The standard interface is C-mount; custom adapters are available on request
How many cameras can run simultaneously?
The SDK supports up to four FL-20BW cameras at once for synchronized imaging across multiple channels
What operating systems are supported?
Windows 7/10 (32 Bit/64 Bit) and Mac. Confirm compatibility with the latest SDK release before ordering
What is the maximum frame rate at full resolution?
8 frames per second at 5472 x 3648 resolution over USB3.0
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