FL-20 Color Cooled SONY CMOS Camera for Fluorescence Imaging
The FL-20 is a 20 megapixel cooled CMOS digital microscope camera with a SONY IMX183CQJ-J sensor, designed for fluorescence imaging and high-resolution sample capture.

Product Overview
The FL-20 is a 20 megapixel cooled CMOS digital microscope camera with a SONY IMX183CQJ-J sensor, designed for fluorescence imaging and high-resolution sample capture. It connects via USB3.0 to a PC and supports real-time image processing, long exposure imaging with low dark current, and simultaneous operation of up to four cameras in a single system. The camera is compatible with standard C-mount microscope optics and delivers 5472 x 3648 resolution at 14 frames per second.
- Sensor TypeCMOS
- Sensor ModelSONY IMX183CQJ-J
- Color/MonoColor
- Array Diagonal15.86mm
- Resolution20MP, 5472(H) x 3648(V)
- Pixel Size2.4μm x 2.4μm
Key Benefits
- 20 megapixel resolution captures full sample detail in a single shot with 5472 x 3648 pixel output
- Cooled CMOS sensor reduces dark current to 0.001e-/pixel/s for clean long-exposure fluorescence imaging
- 14 frames per second at full resolution over USB3.0 enables real-time observation without lag
- Supports 2x2, 3x3, and 4x4 binning to increase frame rate or reduce data load
- Real-time intelligent image processing improves contrast and sharpness without sacrificing speed
- Can operate up to four cameras simultaneously using the SDK for multi-channel imaging setups
- 16-bit or 8-bit output allows flexible data handling for scientific and documentation workflows
Application Scenarios
- Fluorescence imaging in biological or medical research labs requiring long exposure with minimal noise
- High-resolution documentation of microstructures in metallurgy or semiconductor inspection
- Multi-camera imaging setups in microscopy labs using synchronized capture across multiple instruments
- Long-exposure imaging of weakly fluorescent samples where dark current must be minimized
- Teaching or demonstration sessions where real-time image quality and clarity are critical
Who It Is For
- Research and clinical laboratories performing fluorescence microscopy
- Quality control teams in semiconductor and materials science requiring high-resolution imaging
- University teaching labs using digital microscopes for advanced microscopy instruction
- Microscopy system integrators building multi-camera imaging platforms
- Industrial inspection lines needing low-noise, high-resolution capture for defect analysis
Problems It Solves
- Uncooled CMOS cameras generate excessive hot pixels during long exposures, corrupting fluorescence data
- Low frame rates at high resolution make real-time observation difficult during sample alignment
- Lack of binning options limits performance when speed or data volume must be reduced
- Single-camera systems cannot support synchronized capture across multiple microscopes
- Insufficient bit depth or image processing leads to loss of detail in low-contrast samples
Technical Features
- Sony IMX183CQJ-J CMOS sensor with 15.86mm diagonal image area for wide coverage
- 20 megapixel resolution at 5472 x 3648 pixels with 2.4μm x 2.4μm pixel size
- Rolling shutter mode with exposure time range from 18μs to 60 minutes
- Peak quantum efficiency of 84% at 535nm, ideal for green fluorescence detection
- Readout noise below 1 electron, minimizing signal degradation
- Real-time intelligent image processing for enhanced contrast and clarity
- Supports 2x2, 3x3, and 4x4 binning to adjust resolution and frame rate
- USB3.0 interface enables 14fps at full resolution and powers the camera
- C-mount optical interface ensures compatibility with standard microscope objectives
- Operating temperature range 0 degC to 40 degC, humidity 10% to 85% for stable lab use
Specifications
| Sensor Type | CMOS |
|---|---|
| Sensor Model | SONY IMX183CQJ-J |
| Color/Mono | Color |
| Array Diagonal | 15.86mm |
| Resolution | 20MP, 5472(H) x 3648(V) |
| Pixel Size | 2.4μm x 2.4μm |
| Effective Area | 13.1mm x 8.8mm |
| Shutter Mode | Rolling |
| Frame Rate | 14fps @ USB3.0 |
| Peak QE | 84% @ 535nm |
| Readout Noise | <1e |
| Exposure Time | 18μs - 60min |
| Color Temperature | 2000-15000K |
| Binning | 2x2, 3x3, 4x4 |
| PC Software | Mosaic V2 |
| Picture Format | TIFF/JPG/PNG/DICOM |
| Data Interface | USB3.0 |
| Multiple Cameras | Supports 4 cameras simultaneously in SDK |
| SDK | C/C++, C#, Directshow/Twain |
| Optical Interface | Standard C Mount |
| Bit Depth | 16/8bit |
| Power | 12V |
| Operating Environment | Temperature: 0~40 degC, Humidity: 10%~85% |
| Dimensions | 85mm x 85mm x 109mm |
| Camera Weight | 990g |
| Operating System | Windows 7/10 (32 Bit/64 Bit)/Mac |
| PC Configuration | CPU: Intel Core i5 or better (Quad or more Core), RAM: 8G or more |
Specifications are taken from the AMADA product data for this item. Confirm critical dimensions with us in writing before ordering.
Customization Options
- C-mount interface allows direct attachment to standard microscope objectives
- Support for 2x2, 3x3, and 4x4 binning to adjust resolution and frame rate for different workflows
- 16-bit or 8-bit output selection based on data storage and processing needs
- SDK integration allows custom software control for multi-camera or automated imaging systems
- Compatible with PC configurations meeting minimum CPU and RAM requirements
Comparison With Other Options
| Uncooled CMOS microscope camera | The FL-20's cooling reduces dark current to 0.001e-/pixel/s, eliminating hot pixels during long exposures; uncooled sensors show increasing noise over time |
|---|---|
| USB2.0 CMOS camera | The FL-20 uses USB3.0 to deliver 14fps at full 20MP resolution; USB2.0 limits throughput and cannot sustain high frame rates at full resolution |
| Monochrome CMOS camera | The FL-20 is color-sensitive with a full color array, so it captures color information directly; monochrome sensors require filters for color imaging |
Use Guide
- Ensure the microscope objective is mounted with a C-mount adapter before attaching the camera
- Use a PC with at least Intel Core i5 and 8GB RAM to run the Mosaic V2 software and maintain full frame rate
- Enable binning (2x2, 3x3, or 4x4) if you need higher frame rates or reduced data volume
- Set exposure time to 18μs for fast events or up to 60 minutes for long fluorescence exposures
- Use the real-time image processing feature to enhance contrast and sharpness during live viewing
- Connect the camera to a USB3.0 port; the camera draws power from the same cable
- For multi-camera setups, use the SDK to synchronize capture across up to four FL-20 units
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View DetailsFrequently Asked Questions
What is the maximum exposure time?
60 minutes. This allows long exposure imaging for low-light fluorescence samples.
Does the camera require external cooling?
Yes. The FL-20 uses internal cooling technology to reduce dark current to 0.001e-/pixel/s.
Can I use this camera with a stereo or biological microscope?
Yes. As long as the microscope has a C-mount interface, the camera can be attached directly.
What is the frame rate at full resolution?
14 frames per second at 5472 x 3648 pixels over USB3.0.
Can I run multiple FL-20 cameras at once?
Yes. The SDK supports up to four FL-20 cameras operating simultaneously.
Which operating systems are supported?
Windows 7/10 (32 Bit/64 Bit) and Mac. Confirm compatibility with current drivers before ordering.
What software comes with the camera?
Mosaic V2 software is included for image capture, processing, and format export.
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