AMADA INSTRUMENT CO.,LIMITED — Nanjing China
USB3.0 CCD Microscope Camera

FL-20 Color Cooled SONY CMOS Camera for Fluorescence Imaging

The FL-20 is a 20 megapixel cooled CMOS digital microscope camera with a SONY IMX183CQJ-J sensor, designed for fluorescence imaging and high-resolution sample capture.

Product Overview

The FL-20 is a 20 megapixel cooled CMOS digital microscope camera with a SONY IMX183CQJ-J sensor, designed for fluorescence imaging and high-resolution sample capture. It connects via USB3.0 to a PC and supports real-time image processing, long exposure imaging with low dark current, and simultaneous operation of up to four cameras in a single system. The camera is compatible with standard C-mount microscope optics and delivers 5472 x 3648 resolution at 14 frames per second.

  • Sensor TypeCMOS
  • Sensor ModelSONY IMX183CQJ-J
  • Color/MonoColor
  • Array Diagonal15.86mm
  • Resolution20MP, 5472(H) x 3648(V)
  • Pixel Size2.4μm x 2.4μm

Key Benefits

  • 20 megapixel resolution captures full sample detail in a single shot with 5472 x 3648 pixel output
  • Cooled CMOS sensor reduces dark current to 0.001e-/pixel/s for clean long-exposure fluorescence imaging
  • 14 frames per second at full resolution over USB3.0 enables real-time observation without lag
  • Supports 2x2, 3x3, and 4x4 binning to increase frame rate or reduce data load
  • Real-time intelligent image processing improves contrast and sharpness without sacrificing speed
  • Can operate up to four cameras simultaneously using the SDK for multi-channel imaging setups
  • 16-bit or 8-bit output allows flexible data handling for scientific and documentation workflows

Application Scenarios

  • Fluorescence imaging in biological or medical research labs requiring long exposure with minimal noise
  • High-resolution documentation of microstructures in metallurgy or semiconductor inspection
  • Multi-camera imaging setups in microscopy labs using synchronized capture across multiple instruments
  • Long-exposure imaging of weakly fluorescent samples where dark current must be minimized
  • Teaching or demonstration sessions where real-time image quality and clarity are critical

Who It Is For

  • Research and clinical laboratories performing fluorescence microscopy
  • Quality control teams in semiconductor and materials science requiring high-resolution imaging
  • University teaching labs using digital microscopes for advanced microscopy instruction
  • Microscopy system integrators building multi-camera imaging platforms
  • Industrial inspection lines needing low-noise, high-resolution capture for defect analysis

Problems It Solves

  • Uncooled CMOS cameras generate excessive hot pixels during long exposures, corrupting fluorescence data
  • Low frame rates at high resolution make real-time observation difficult during sample alignment
  • Lack of binning options limits performance when speed or data volume must be reduced
  • Single-camera systems cannot support synchronized capture across multiple microscopes
  • Insufficient bit depth or image processing leads to loss of detail in low-contrast samples

Technical Features

  • Sony IMX183CQJ-J CMOS sensor with 15.86mm diagonal image area for wide coverage
  • 20 megapixel resolution at 5472 x 3648 pixels with 2.4μm x 2.4μm pixel size
  • Rolling shutter mode with exposure time range from 18μs to 60 minutes
  • Peak quantum efficiency of 84% at 535nm, ideal for green fluorescence detection
  • Readout noise below 1 electron, minimizing signal degradation
  • Real-time intelligent image processing for enhanced contrast and clarity
  • Supports 2x2, 3x3, and 4x4 binning to adjust resolution and frame rate
  • USB3.0 interface enables 14fps at full resolution and powers the camera
  • C-mount optical interface ensures compatibility with standard microscope objectives
  • Operating temperature range 0 degC to 40 degC, humidity 10% to 85% for stable lab use

Specifications

Sensor TypeCMOS
Sensor ModelSONY IMX183CQJ-J
Color/MonoColor
Array Diagonal15.86mm
Resolution20MP, 5472(H) x 3648(V)
Pixel Size2.4μm x 2.4μm
Effective Area13.1mm x 8.8mm
Shutter ModeRolling
Frame Rate14fps @ USB3.0
Peak QE84% @ 535nm
Readout Noise<1e
Exposure Time18μs - 60min
Color Temperature2000-15000K
Binning2x2, 3x3, 4x4
PC SoftwareMosaic V2
Picture FormatTIFF/JPG/PNG/DICOM
Data InterfaceUSB3.0
Multiple CamerasSupports 4 cameras simultaneously in SDK
SDKC/C++, C#, Directshow/Twain
Optical InterfaceStandard C Mount
Bit Depth16/8bit
Power12V
Operating EnvironmentTemperature: 0~40 degC, Humidity: 10%~85%
Dimensions85mm x 85mm x 109mm
Camera Weight990g
Operating SystemWindows 7/10 (32 Bit/64 Bit)/Mac
PC ConfigurationCPU: Intel Core i5 or better (Quad or more Core), RAM: 8G or more

Specifications are taken from the AMADA product data for this item. Confirm critical dimensions with us in writing before ordering.

Customization Options

  • C-mount interface allows direct attachment to standard microscope objectives
  • Support for 2x2, 3x3, and 4x4 binning to adjust resolution and frame rate for different workflows
  • 16-bit or 8-bit output selection based on data storage and processing needs
  • SDK integration allows custom software control for multi-camera or automated imaging systems
  • Compatible with PC configurations meeting minimum CPU and RAM requirements

Comparison With Other Options

Uncooled CMOS microscope cameraThe FL-20's cooling reduces dark current to 0.001e-/pixel/s, eliminating hot pixels during long exposures; uncooled sensors show increasing noise over time
USB2.0 CMOS cameraThe FL-20 uses USB3.0 to deliver 14fps at full 20MP resolution; USB2.0 limits throughput and cannot sustain high frame rates at full resolution
Monochrome CMOS cameraThe FL-20 is color-sensitive with a full color array, so it captures color information directly; monochrome sensors require filters for color imaging

Use Guide

  • Ensure the microscope objective is mounted with a C-mount adapter before attaching the camera
  • Use a PC with at least Intel Core i5 and 8GB RAM to run the Mosaic V2 software and maintain full frame rate
  • Enable binning (2x2, 3x3, or 4x4) if you need higher frame rates or reduced data volume
  • Set exposure time to 18μs for fast events or up to 60 minutes for long fluorescence exposures
  • Use the real-time image processing feature to enhance contrast and sharpness during live viewing
  • Connect the camera to a USB3.0 port; the camera draws power from the same cable
  • For multi-camera setups, use the SDK to synchronize capture across up to four FL-20 units
Answers

Frequently Asked Questions

What is the maximum exposure time?

60 minutes. This allows long exposure imaging for low-light fluorescence samples.

Does the camera require external cooling?

Yes. The FL-20 uses internal cooling technology to reduce dark current to 0.001e-/pixel/s.

Can I use this camera with a stereo or biological microscope?

Yes. As long as the microscope has a C-mount interface, the camera can be attached directly.

What is the frame rate at full resolution?

14 frames per second at 5472 x 3648 pixels over USB3.0.

Can I run multiple FL-20 cameras at once?

Yes. The SDK supports up to four FL-20 cameras operating simultaneously.

Which operating systems are supported?

Windows 7/10 (32 Bit/64 Bit) and Mac. Confirm compatibility with current drivers before ordering.

What software comes with the camera?

Mosaic V2 software is included for image capture, processing, and format export.

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