F180 Inverted Fluorescent Semiapochromat Biological Phase Contrast Objective

Key Benefits

• Semiapochromat correction reduces chromatic and spherical aberration for clearer images across the visible spectrum
• Phase contrast capability enhances contrast in transparent or low-contrast biological specimens without staining
• 45mm field number ensures a large, consistent field of view across all magnifications
• 45mm parfocal length allows quick switching between objectives without refocusing
• Inverted design enables observation of specimens in culture dishes, flasks, and other containers from below
• Working distances from 18mm (4X) to 2mm (60X) accommodate various sample thicknesses and container types

Application Scenarios

• Observing live cell cultures in petri dishes or flasks on an inverted fluorescence microscope
• Inspecting tissue sections or fixed cells in culture with phase contrast enhancement
• Performing long-term time-lapse imaging of growing or dividing cells in a controlled environment
• Conducting quality control on cell lines in biopharmaceutical or research labs
• Documenting cellular morphology and structural changes in developmental biology studies

Who It Is For

• Biological and medical research laboratories studying live or fixed cells
• Clinical and veterinary diagnostic labs using phase contrast for specimen analysis
• Pharmaceutical and biotech labs monitoring cell culture health and growth
• University teaching labs demonstrating phase contrast and fluorescence microscopy
• Industrial quality control labs inspecting biological materials in production

Problems It Solves

• Standard objectives fail to resolve fine cellular structures in transparent specimens without staining
• Mismatched parfocal length between objectives causes refocusing after each magnification change
• Short working distances in high-magnification objectives prevent observation of thick or deep samples
• Chromatic aberration in low-cost objectives reduces image clarity and contrast
• Incompatible mounting systems prevent use across different microscope models

Technical Features

• Inverted fluorescent semiapochromat design for use on inverted microscopes
• Semiapochromat correction for reduced chromatic and spherical aberration
• Phase contrast capability for enhanced contrast in transparent specimens
• 45mm field number ensures consistent field size across all magnifications
• 45mm parfocal length allows objective switching without refocusing
• Working distances range from 18mm (4X) to 2mm (60X) for flexible sample accommodation
• Numerical aperture values from 0.15 (4X) to 0.75 (60X) support high-resolution imaging
• Compatible with both inverted and upright fluorescence systems

Specifications

Specifications are taken from the AMADA product data for this item. Confirm critical dimensions with us in writing before ordering.

Customization Options

• Available in 4X, 10X, 20X, 40X, and 60X magnifications with phase contrast capability
• Compatible with standard C-mount inverted microscope bodies
• Can be used in both inverted and upright fluorescence systems with proper alignment

Comparison With Other Options

Use Guide

• Ensure the microscope stage is properly aligned and the condenser is set for phase contrast before use
• Use the correct phase ring in the condenser to match the objective's phase contrast design
• Start at low magnification (4X) to locate the specimen, then switch to higher magnifications
• Adjust the condenser height and aperture to optimize contrast and resolution
• Clean the objective lens with lens paper and ethanol before and after use to maintain optical clarity