AMADA INSTRUMENT CO.,LIMITED — Nanjing China
Inverted Fluorescence Microscope

XIF200 Inverted Research Fluorescence Microscope

The XIF200 is an inverted research fluorescence microscope with an infinity independent achromatic optical system and a one-piece structure for stability.

Product Overview

The XIF200 is an inverted research fluorescence microscope with an infinity independent achromatic optical system and a one-piece structure for stability. It features a binocular inclined 45o head with interpupillary adjustment from 48mm to 75mm, a high eye-point plan eyepiece WF10X/20, and a range of infinity long distance plan achromatic objectives from 4X to 60X. The system supports fluorescence excitation with UV, blue, and green filters, phase-contrast objectives, and a mechanical stage with 50mm x 50mm movement. It is designed for live cell imaging, tissue culture, and material analysis under fluorescence and phase-contrast conditions.

  • Optical systemInfinity Independent Achromatic
  • HeadBinocular, Inclined 45o
  • Interpupillary adjustment48-75mm
  • EyepieceWF10X/20
  • Objective4X, 10X, 20X, 40X, 60X
  • Phase-contrast objectivesPositive and negative contrast available

Key Benefits

  • Infinity optical system ensures consistent image quality across all magnifications and objectives
  • Inverted design allows observation of specimens on a large stage with minimal interference from the optics
  • Binocular head with 45o inclination and 48-75mm interpupillary adjustment improves operator comfort during long sessions
  • High eye-point eyepiece WF10X/20 enables comfortable viewing with glasses and reduces eye strain
  • Supports multiple fluorescence excitation filters (UV, blue, green) and phase-contrast objectives for diverse sample types
  • Mechanical stage with 50mm x 50mm movement range allows precise positioning of large or multiple samples
  • Ultra long working distance condenser supports thick specimens and high numerical aperture objectives
  • C-mount adapters (0.5X, 0.25X, 1X) allow integration with external cameras for documentation and analysis

Application Scenarios

  • Live cell imaging in tissue culture labs where the inverted design avoids interference with culture dishes
  • Fluorescence inspection of biological samples such as stained cells, tissues, or proteins
  • Phase-contrast observation of unstained live cells and microorganisms in liquid culture
  • Material science analysis of thin films, coatings, or semiconductor layers under fluorescence
  • Quality control of microelectronic components using fluorescence to detect defects or contamination
  • Teaching and demonstration in university labs where multiple students observe the same sample via camera output

Who It Is For

  • Research laboratories in biology, cell biology, and biomedical sciences
  • Clinical and veterinary diagnostic labs performing fluorescence staining and cell analysis
  • Material science and semiconductor quality control departments
  • University teaching labs in life sciences and engineering
  • Industrial R&D teams working with biological or optical materials

Problems It Solves

  • Traditional upright microscopes obstruct the space above the specimen, limiting access for culture dishes or large samples
  • Fixed interpupillary distance forces operators to adjust head position, causing fatigue during extended use
  • Lack of fluorescence excitation filters and phase-contrast objectives limits the ability to analyze live or unstained samples
  • Small mechanical stage movement range restricts the ability to scan large or multiple samples without repositioning
  • No C-mount interface prevents integration with digital cameras for documentation or automated analysis
  • Poor optical stability in multi-objective systems leads to focus shift and image degradation

Technical Features

  • Infinity independent achromatic optical system for consistent image quality across objectives
  • One-piece structure enhances mechanical stability and reduces vibration during observation
  • Binocular inclined 45o head with interpupillary adjustment 48-75mm and diopter adjustment
  • High eye-point plan eyepiece WF10X/20 for comfortable viewing with glasses
  • Infinity long distance plan achromatic objectives: 4X, 10X, 20X, 40X, 60X
  • Phase-contrast objectives available in positive and negative contrast modes
  • UV, blue, and green excitation filters for fluorescence imaging
  • Multi-functional semi-reflector for switching between brightfield and fluorescence
  • Fixed stage 240mm x 260mm with XY hand wheel movement range 135mm x 85mm
  • Mechanical stage 350mm x 208mm with 50mm x 50mm movement range
  • Quintuple nosepiece for quick objective switching
  • Ultra long working distance condenser for thick specimens and high NA objectives
  • LED lamp house and 6V 30W halogen lamp with Osram bulb for stable illumination
  • 100W DC Osram mercury lamp house and power supply for high-intensity fluorescence
  • C-mount adapters (0.5X, 0.25X, 1X) for camera integration
  • Filter holders with blue, green, yellow filters (φ34mm)
  • Diaphragm adjustment handle for controlling illumination intensity
  • Dust cover for protection during storage

Specifications

Optical systemInfinity Independent Achromatic
HeadBinocular, Inclined 45o
Interpupillary adjustment48-75mm
EyepieceWF10X/20
Objective4X, 10X, 20X, 40X, 60X
Phase-contrast objectivesPositive and negative contrast available
Excitation filtersUV, Blue, Green
CondenserUltra Long WD
Stage typeFixed and Mechanical
Fixed stage size240mm x 260mm
Fixed stage movement135mm x 85mm
Mechanical stage size350mm x 208mm
Mechanical stage movement50mm x 50mm
NosepieceQuintuple
C-mount adapters0.5X, 0.25X, 1X
Illumination sourceLED, 6V 30W Halogen, 100W DC Mercury
Lamp power6V 30W Halogen, 100W DC Mercury
Filter sizeφ34mm
DiaphragmAdjustable
Dust coverIncluded
Power supply250V/5A Φ5X20 fuse, 250V/3.15A Φ5X20 fuse

Specifications are taken from the AMADA product data for this item. Confirm critical dimensions with us in writing before ordering.

Customization Options

  • Binocular head with optional diopter adjustment for individual operator settings
  • Selection of eyepieces: WF10X/20, WF10X/22, WF10X/20 reticule
  • Choice of objectives: 4X, 10X, 20X, 40X, 60X, phase-contrast variants
  • Addition of UV, blue, green excitation filters and corresponding filter holders
  • Integration of 100W DC Osram mercury lamp house and power supply
  • Installation of C-mount adapters (0.5X, 0.25X, 1X) for camera connection
  • Replacement of standard halogen lamp with 100W DC mercury lamp for high-intensity fluorescence
  • Addition of multi-functional specimen holder (76x26 φ54mm) for specialized samples

Comparison With Other Options

XIF200 without fluorescence filtersThe standard XIF200 includes UV, blue, and green excitation filters; the non-fluorescence version lacks these and cannot perform fluorescence imaging.
XIF200 with upright designThe inverted design allows access to large or thick specimens on the stage; upright models obstruct the space above the sample.
XIF200 with fixed stage onlyThe XIF200 includes a mechanical stage with 50mm x 50mm movement; the fixed stage version requires manual repositioning for large samples.

Use Guide

  • Adjust the interpupillary distance between 48mm and 75mm before focusing to ensure a single merged image
  • Use the diopter adjustment on the right eyepiece to match individual vision before focusing
  • Start with the lowest magnification objective (4X) to locate the specimen, then switch to higher magnifications
  • For fluorescence imaging, ensure the correct excitation filter is in place and the mercury lamp is properly aligned
  • Use the mechanical stage for precise movement of large or multiple samples without repositioning the specimen
  • Connect a camera via the 0.5X, 0.25X, or 1X C-mount adapter for image capture and documentation
  • Allow the mercury lamp to warm up for at least 10 minutes before use to achieve stable output
  • Cover the microscope with the dust cover when not in use to prevent contamination
Read Next

Guides and Applications

Answers

Frequently Asked Questions

What is the working distance for the 40X and 60X objectives?

The infinity long distance plan objectives are designed for high working distance; specific values are not listed in the source text.

Can I use this microscope for live cell imaging?

Yes. The inverted design and stable illumination allow observation of live cells in culture dishes without interference from the optics.

Does the microscope support phase-contrast imaging?

Yes. Phase-contrast objectives are available in positive and negative contrast modes for observing unstained specimens.

What camera adapters are included?

The microscope includes 0.5X, 0.25X, and 1X C-mount adapters for connecting external cameras.

Can I upgrade to a mercury lamp?

Yes. The 100W DC Osram mercury lamp house and power supply are available as an option for high-intensity fluorescence imaging.

Is the UV filter included?

Yes. The UV excitation filter is included in the standard configuration.

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