AMADA INSTRUMENT CO.,LIMITED — Nanjing China
Inverted Fluorescence Microscope

XIF100 Inverted Research Fluorescence Microscope

The XIF100 is an inverted research fluorescence microscope with an infinity independent achromatic optical system and one-piece structure for stability.

Product Overview

The XIF100 is an inverted research fluorescence microscope with an infinity independent achromatic optical system and one-piece structure for stability. It supports binocular or trinocular heads with interpupillary adjustment from 48mm to 75mm and offers a range of objectives from 4X to 60X, including phase-contrast and fluorescence options. The system includes a built-in fluorescence illuminator, filter sets for UV, blue, and green excitation, and C-mount adapters for digital cameras, enabling live imaging and documentation of fluorescent specimens on a flat stage.

  • Optical systemInfinity Independent Achromatic
  • Head typeBinocular / Trinocular
  • Interpupillary adjustment48-75mm
  • Eyepiece field number18mm, 20mm, 22mm
  • Eyepiece magnificationWF10X, WF16X
  • Objective magnification4X, 10X, 20X, 40X, 60X

Key Benefits

  • Infinity optical system ensures consistent image quality across all magnifications and objectives
  • One-piece structure provides mechanical stability for long-term research and repeated use
  • Binocular or trinocular head with 48-75mm interpupillary adjustment supports multiple users and camera integration
  • Fluorescence illuminator with UV, blue, and green excitation filters enables multi-channel imaging
  • C-mount adapters (0.25X, 1X, 0.5X) allow connection to 1/3 CCD, 1/2", and 2.5X digital cameras
  • Mechanical stage with 180mm x 155mm travel range and 96-well plate compatibility supports high-throughput screening
  • Phase-contrast objectives (positive and negative) allow contrast enhancement in transparent specimens
  • High eye-point plan eyepieces reduce eye strain during extended observation sessions

Application Scenarios

  • Fluorescent cell culture imaging in a university or research lab with live or fixed samples
  • High-throughput screening of 96-well plates in pharmaceutical or biotech quality control
  • Phase-contrast and fluorescence imaging of microorganisms in microbiology labs
  • Documentation of fluorescently labeled tissue sections with a digital camera attached via C-mount
  • Teaching advanced microscopy techniques in a university laboratory with multiple observers

Who It Is For

  • University and research laboratories conducting fluorescence and phase-contrast microscopy
  • Biotech and pharmaceutical labs performing cell culture and assay screening
  • Clinical and veterinary labs analyzing fluorescently stained samples
  • Teaching labs in biology and life sciences departments
  • Quality control departments in biotechnology and medical device manufacturing

Problems It Solves

  • A fixed-stage microscope limits the size and type of specimen holders that can be used
  • Lack of phase-contrast objectives makes transparent or low-contrast specimens difficult to observe
  • No built-in fluorescence illuminator forces the buyer to source and integrate a separate light source
  • Inconsistent optical performance across magnifications due to non-infinity systems
  • No C-mount compatibility prevents direct connection to digital cameras for documentation
  • Fixed interpupillary distance makes the microscope unsuitable for users with different eye spacing

Technical Features

  • Infinity independent achromatic optical system with long working distance objectives
  • One-piece structural design for mechanical stability and long-term alignment
  • Binocular or trinocular head with interpupillary adjustment 48-75mm and diopter compensation
  • High eye-point plan eyepieces with field numbers 18mm, 20mm, and 22mm for comfortable viewing
  • Infinity long distance plan achromatic objectives: 4X, 10X, 20X, 40X, 60X
  • Phase-contrast objectives available in positive and negative contrast modes
  • Inverted fluorescence illuminator with UV, blue, and green excitation filters
  • C-mount adapters: 0.25X, 1X, 0.5X for 1/3 CCD, 1/2", and 2.5X digital cameras
  • Mechanical stage with 180mm x 155mm travel range and 96-well plate holder
  • LED light source and 6V 30W halogen lamp house for illumination flexibility
  • Filter holder with blue, green, and yellow filters (φ34) for spectral control
  • Specimen holder (φ65), drop holder (φ110), and multi-functional holder (φ54) for varied sample types

Specifications

Optical systemInfinity Independent Achromatic
Head typeBinocular / Trinocular
Interpupillary adjustment48-75mm
Eyepiece field number18mm, 20mm, 22mm
Eyepiece magnificationWF10X, WF16X
Objective magnification4X, 10X, 20X, 40X, 60X
Objective typeInfinity Long Distance Plan Achromatic, Phase-contrast (positive/negative)
Fluorescence excitationUV, Blue, Green
Illumination sourceLED, 6V 30W Halogen, 100W DC Me
C-mount adapters0.25X, 1X, 0.5X
Camera sensor size1/3", 1/2", 2.5X
Stage typeFixed + Attachable Mechanical Stage
Stage travel range80mm x 50mm
Specimen holder diameterφ65, φ54, φ35, φ110
96-well plate compatibilityYes, with mechanical stage
Filter sizeφ34
Filter typesBlue, Green, Yellow
Reticle eyepieceWF10X/18mm, WF10X/20mm
NosepieceQuintuple
Light source power6V 30W, 100W DC Me

Specifications are taken from the AMADA product data for this item. Confirm critical dimensions with us in writing before ordering.

Customization Options

  • Binocular or trinocular head configuration on the same XIF100 body
  • Selection of high eye-point plan eyepieces (WF10X/18mm, WF10X/20mm, WF10X/22mm)
  • Addition of reticle eyepieces for measurement or calibration
  • Optional phase-contrast objectives (positive or negative) for transparent specimen contrast
  • C-mount adapter upgrade to 0.25X, 1X, or 0.5X for different camera sensor sizes
  • Mechanical stage with 96-well plate holder for high-throughput applications
  • Fluorescence filter set upgrade to include UV, blue, and green excitation filters

Comparison With Other Options

XIF100 without fluorescence illuminatorThe XIF100 UCIS includes a built-in fluorescence illuminator with UV, blue, and green excitation filters, so no separate light source is needed for fluorescent imaging.
Standard upright fluorescence microscopeThe inverted design allows large or thick specimens, such as cell cultures in Petri dishes, to be viewed from below without obstruction.
Microscope with fixed interpupillary distanceThe XIF100 offers 48-75mm interpupillary adjustment, so it can be shared between users with different eye spacing without repositioning.

Use Guide

  • Adjust the interpupillary distance to match your eye spacing before focusing to avoid eye strain
  • Use the diopter adjustment on the eyepiece to fine-tune focus for each eye
  • Select the appropriate excitation filter (UV, blue, green) based on your fluorophore
  • Place the specimen on the mechanical stage and secure it with the specimen clip or holder
  • Use the 96-well plate holder for high-throughput screening applications
  • Connect the camera via the 0.25X, 1X, or 0.5X C-mount adapter depending on sensor size
  • Ensure the halogen lamp is properly seated and the power supply matches the input voltage
Read Next

Guides and Applications

Answers

Frequently Asked Questions

What is the maximum magnification with the 60X objective?

The maximum magnification is 600X when used with a WF10X eyepiece.

Can I use this microscope for live cell imaging?

Yes. The inverted design and stable structure allow for long-term observation of live cells in culture dishes on the stage.

Does the fluorescence illuminator require a separate power supply?

No. The illuminator is powered through the main unit's AC 100-240V supply.

What camera sensors are compatible with the C-mount adapters?

The 0.25X adapter fits 1/3" CCD sensors, the 1X fits 1/2" sensors, and the 0.5X fits 2.5X digital cameras.

Can I switch between phase-contrast and brightfield imaging?

Yes. The microscope includes phase-contrast objectives and a phase-contrast slide for switching between modes.

Is the mechanical stage compatible with 96-well plates?

Yes. The 96-well plate mechanical stage has a travel range of 80mm x 50mm and is designed for standard 96-well plates.

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