Blotting Membrane Filter product photo 1

Membrane Filter

Blotting Membrane Filter

The Blotting Membrane Filter is a nitrocellulose-based membrane designed for high-efficiency protein and nucleic acid transfer in Western blot, dot-blot, and related assays. Available in 0.22 μm and 0.45 μm pore sizes, the membrane offers high binding capacity, uniform surface structure, and low background. Rolls are supplied in 300 x 3000 mm format for use in semi-dry and wet transfer systems. The membrane is suitable for stripping and reprobing applications, supporting repeated use in research workflows.

100% pure nitrocellulose ensures minimal interference in protein and nucleic acid detection
High protein binding capacity supports strong signal intensity in low-abundance targets
Uniform surface structure and consistent thickness (115 μm ±20) improve transfer reproducibility
Low background reduces false positives in detection, especially in chemiluminescent and colorimetric assays

Key Benefits

What This Product Gives You

100% pure nitrocellulose ensures minimal interference in protein and nucleic acid detection
High protein binding capacity supports strong signal intensity in low-abundance targets
Uniform surface structure and consistent thickness (115 μm ±20) improve transfer reproducibility
Low background reduces false positives in detection, especially in chemiluminescent and colorimetric assays
Available in 0.22 μm and 0.45 μm pore sizes to match target molecule size and transfer efficiency

Material: 100% pure nitrocellulose
Pore sizes: 0.22 μm and 0.45 μm
Thickness: 115 μm ±20
Bubble point with water: 0.23-0.26 MPa (0.22 μm), 0.4-0.5 MPa (0.45 μm)
Flow rate for water: >50 ml/(min·cm²·bar) (0.22 μm), >30 ml/(min·cm²·bar) (0.45 μm)
Wetting time with water: <1 second
Format: Roll, 300 mm x 3000 mm
High surface area and uniformity for consistent transfer

Where It Is Used

Applications And Buyers

Application Scenarios

Transferring proteins from SDS-PAGE gels to membrane for Western blot analysis
Performing dot-blot assays for antigen detection in serum or cell lysates
Conducting nucleic acid hybridization with low molecular weight DNA or RNA
Supporting stripping and reprobing workflows in multi-target protein analysis
Using in semi-dry or wet transfer systems for consistent and even transfer

Who It Is For

Life-science research laboratories conducting protein and nucleic acid detection
Academic and contract research labs performing Western blot and dot-blot experiments
OEM partners developing blotting kits requiring consistent membrane performance
Laboratories needing reproducible transfer results across multiple runs and operators

Problems It Solves

Low protein binding leading to weak or undetectable signals in Western blot
Inconsistent transfer efficiency due to non-uniform membrane thickness or pore size
High background noise from impurities or non-specific binding in detection
Membrane degradation during stripping or reprobing due to poor material stability
Inability to reuse membranes for multiple rounds of detection without signal loss

Data

Specifications

Material	100% pure nitrocellulose
Pore Size	0.22 μm, 0.45 μm
Thickness	115 μm ±20
Bubble Point (Water)	0.23-0.26 MPa (0.22 μm), 0.4-0.5 MPa (0.45 μm)
Flow Rate (Water)	>50 ml/(min·cm²·bar) (0.22 μm), >30 ml/(min·cm²·bar) (0.45 μm)
Wetting Time (Water)	<1 second
Format	Roll, 300 mm x 3000 mm
Binding Capacity	High protein binding
Reprobing Support	Yes, for stripping and reprobing applications

Materials

100% pure nitrocellulose membrane
No additives or synthetic polymers

Customization Options

Available in 0.22 μm and 0.45 μm pore sizes
Roll format: 300 mm x 3000 mm standard
OEM private-label printing and packaging available through Microlab OEM division
Special formats for stripping and reprobing can be developed upon request

How It Is Made

Manufacturing And Quality Control

Manufacturing Process

Nitrocellulose membrane production from high-purity raw material
Roll casting and drying to achieve uniform thickness and surface structure
Pore size control via filtration and calibration
Wetting and bubble point testing for quality verification
Cutting and packaging of rolls in 300 x 3000 mm format

Quality Control

Thickness measured across multiple points to ensure 115 μm ±20 tolerance
Bubble point tested per pore size to confirm integrity and pore uniformity
Wetting time verified to be under 1 second with water
Visual inspection for surface defects, tears, or non-uniformity
Batch traceability maintained for reproducibility across orders

Comparison

Compared With The Alternatives

Versus Mixed cellulose ester (MCE) membranes

Nitrocellulose has higher protein binding and lower background than MCE, but MCE may offer better mechanical strength for handling

Versus Pre-coated membranes

The pure nitrocellulose membrane allows full control over blocking and detection steps, unlike pre-coated membranes that limit method flexibility

Versus Sheet formats

Roll format enables continuous use in semi-dry transfer systems and reduces waste compared to pre-cut sheets

Use Guide

How To Use It

Unroll the membrane carefully to avoid creasing or tearing
Soak the membrane in transfer buffer for 1-2 minutes before use to ensure even wetting
Use 0.22 μm for small proteins and 0.45 μm for larger proteins or nucleic acids
Place the membrane between the gel and the transfer stack, ensuring no air bubbles
Perform transfer in semi-dry or wet system according to protocol
After transfer, block the membrane with appropriate blocking agent before antibody incubation
For reprobing, strip the membrane with recommended buffer and re-block before re-detection

FAQ

Questions Buyers Ask

What is the difference between 0.22 μm and 0.45 μm pore sizes?

The 0.22 μm pore size retains smaller proteins and nucleic acids, while 0.45 μm is better suited for larger proteins and allows faster transfer

Can this membrane be used for reprobing?

Yes, the membrane supports stripping and reprobing, making it suitable for multiple rounds of detection

What format is the membrane supplied in?

The membrane is supplied as a roll, 300 mm x 3000 mm, for use in semi-dry and wet transfer systems

Is the membrane compatible with chemiluminescent detection?

Yes, the low background and high binding capacity make it suitable for chemiluminescent and colorimetric detection methods

Can I get custom roll sizes?

Yes, special formats and roll dimensions can be developed through the Microlab OEM division

Do you offer private-label packaging?

Yes, the OEM division supports private-label printing and packaging for branded kits or reagents

Blotting Membrane Filter

What This Product Gives You

Applications And Buyers

Application Scenarios

Who It Is For

Problems It Solves

Specifications

Materials

Customization Options

Manufacturing And Quality Control

Manufacturing Process

Quality Control

Compared With The Alternatives

Versus Mixed cellulose ester (MCE) membranes

Versus Pre-coated membranes

Versus Sheet formats

How To Use It

Product Gallery

Related Products

RC Membrane Filter

PVDF Membrane Filter

PTFE Membrane Filter

PP Membrane Filter

Related Industries And Guides

Questions Buyers Ask

Get A Quote For Blotting Membrane Filter