AMADA INSTRUMENT CO.,LIMITED — Nanjing China
Upright Fluorescence Microscope

XUF100 Upright Research Fluorescence Microscope

The XUF100 is an upright research fluorescence microscope with a one-piece frame and a choice of binocular or trinocular observation tubes.

Product Overview

The XUF100 is an upright research fluorescence microscope with a one-piece frame and a choice of binocular or trinocular observation tubes. It supports 2X to 100X magnification with infinity plan achromatic objectives and includes a Kohler illuminator with field diaphragm. The fluorescence attachment accepts imported UV, V, B, and G excitation filters. It is designed for biological and material research requiring high-resolution imaging under fluorescence illumination.

  • Optical systemInfinity plan achromatic
  • Head typeBinocular or Trinocular
  • Interpupillary adjustment48-75mm or 52-75mm
  • Tube inclination30°
  • Rotation360°
  • Diopter adjustmentYes

Key Benefits

  • One-piece frame ensures stability during long observation sessions
  • Trinocular tube with 20/80 light split allows simultaneous viewing and camera capture
  • Infinity plan achromatic objectives deliver flat, high-contrast images across the field
  • Kohler illuminator with field diaphragm provides even illumination and reduces glare
  • Multiple objective choices from 2X to 100X (oil immersion) support diverse sample types
  • Interchangeable halogen and LED light sources offer flexibility in power and heat output

Application Scenarios

  • Fluorescence imaging of stained tissue sections in a clinical or research lab
  • Live cell imaging with low-heat LED illumination to avoid sample damage
  • Material surface analysis using fluorescent dyes or coatings
  • Microbial colony detection under UV or blue excitation
  • Documentation of fluorescence patterns with a camera via C-mount adapter

Who It Is For

  • Research laboratories in biology, medicine, and materials science
  • Clinical pathology labs performing fluorescence-based diagnostics
  • University teaching labs requiring stable, configurable fluorescence setups
  • Quality control teams inspecting coated or doped materials
  • Microscopy labs conducting long-duration imaging sessions

Problems It Solves

  • A fixed observation tube limits ergonomic access for multiple users or camera integration
  • Poor illumination uniformity causes uneven brightness and reduces image quality
  • Lack of a trinocular port forces users to choose between direct viewing and imaging
  • Incompatible light sources require separate purchases and power supplies
  • Non-infinity optics introduce image distortion at high magnification

Technical Features

  • One-piece construction from arm to base for mechanical stability
  • Binocular or trinocular observation tube with 30° inclination and 360° rotation
  • Interpupillary adjustment range of 48-75mm or 52-75mm depending on tube type
  • Diopter adjustment for each eye to accommodate individual vision differences
  • Infinity plan achromatic objectives with N.A. from 0.06 to 1.25
  • Kohler illuminator with field diaphragm for uniform illumination
  • Fluorescence attachment with support for imported UV, V, B, and G excitation filters
  • Mechanical stage with 156x138mm or 185x177mm movement range
  • Revolving nosepiece with quadruple or quintuple objective positions
  • Multiple light source options: 6V20W halogen, 6V30W halogen, or 3W LED with rechargeable battery

Specifications

Optical systemInfinity plan achromatic
Head typeBinocular or Trinocular
Interpupillary adjustment48-75mm or 52-75mm
Tube inclination30°
Rotation360°
Diopter adjustmentYes
Objective magnification2X, 4X, 10X, 20X(S), 40X(S), 60X(S), 100X(S) (Oil)
Objective N.A.0.06, 0.10, 0.25, 0.40, 0.65, 0.80, 1.25
CondenserAbbe Condenser N.A.1.25, Swing-out Condenser
Stage typeMechanical Stage
Stage size156x138mm or 185x177mm
Stage movement range76x54mm or 75x50mm
NosepieceQuadruple inward facing or Quintuple inward facing
IlluminatorKohler with field diaphragm
Fluorescence excitation filtersUV, V, B, G (imported)
Light source6V20W Halogen, 6V30W Halogen, 3W LED (with rechargeable battery)
Bulb type6V20W Osram Halogen, 6V30W Osram Halogen, 3W LED
Fuse rating250V/3.15A Φ5X20
Rechargeable battery2200mAh LED
C-mount adapter0.5X, 1X, 0.25X
Filter diameterφ38
Filter typesBlue, Green, Yellow, Frosted glass

Specifications are taken from the AMADA product data for this item. Confirm critical dimensions with us in writing before ordering.

Customization Options

  • Choose between binocular or trinocular observation tubes
  • Select trinocular tube with 20/80, 50/50, or 100/0 light split
  • Add optional objectives: 2X, 20X(S), 40X(S), 60X(S), 100X(S) (Oil)
  • Upgrade to 6V30W halogen or 3W LED with rechargeable battery
  • Add C-mount adapters: 0.5X, 1X, 0.25X for camera integration
  • Include optional filters: Blue, Green, Yellow, Frosted glass (φ38)

Comparison With Other Options

XUF101 upright fluorescence microscopeThe XUF100 offers a wider range of objective options and supports both 20/80 and 50/50 trinocular light splits; the XUF101 is limited to 100/0 split only.
Standard upright biological microscopeThe XUF100 includes a Kohler illuminator and fluorescence attachment, which are not standard on basic biological models.
Digital fluorescence microscopeThe XUF100 uses a traditional optical path with C-mount adaptability, so it supports external cameras and does not rely on built-in digital sensors or software.

Use Guide

  • Adjust the interpupillary distance to match your eye spacing before focusing
  • Use the Kohler illuminator to align the light source and condenser for even illumination
  • For oil immersion, apply a drop of immersion oil to the 100X objective and clean it after use
  • Select the appropriate excitation filter (UV, V, B, G) based on your fluorophore
  • Use the 20/80 trinocular split when capturing images with a camera while maintaining a clear view
  • Ensure the stage is locked when moving the microscope or transporting it
Read Next

Guides and Applications

Answers

Frequently Asked Questions

What is the maximum magnification with the 100X objective?

100X with the 100X(S) objective; total magnification depends on the eyepiece used (e.g., WF10X/20 gives 1000X total).

Can I use this microscope with a digital camera?

Yes. Use the 1X C-mount adapter to connect a digital camera to the trinocular port.

Does the fluorescence attachment include filters?

The attachment supports imported UV, V, B, and G excitation filters; the filters are not included with the microscope.

What light source options are available?

6V20W halogen, 6V30W halogen, or 3W LED with rechargeable battery; the 3W LED includes a 2200mAh battery.

Is the 100X objective oil immersion?

Yes. The 100X(S) objective requires immersion oil for proper use and maximum resolution.

Can I switch between binocular and trinocular heads?

Yes. The XUF100 body supports both binocular and trinocular head configurations; specify your choice when ordering.

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