Syringe Filter

BioFil Syringe Filter

BioFil™ syringe filters are designed for high-particulate samples where standard filters clog quickly. The multi-layer membrane structure includes a PP or GF prefilter, extending filter life and reducing the risk of column blockage during routine sample preparation. Available in 4 mm, 13 mm, and 25 mm diameters with nine membrane types, the filter family supports aqueous, organic, and protein-containing matrices. Sealing is achieved via ultrasonic welding, which maintains low extractables and ensures integrity under pressure. The filters are supplied in 100-piece packs with part numbers and membrane type printed directly on the housing.

Multi-layer membrane with PP or GF prefilter extends life when filtering samples with high particulate loads
Ultrasonic welding sealing avoids adhesive residues, reducing extractables in sensitive HPLC and GC methods
Available in 4 mm, 13 mm, and 25 mm diameters to match sample volume and particle load requirements
Nine membrane types cover aqueous, organic, and protein-based samples, reducing the need for multiple filter types

Key Benefits

What This Product Gives You

Multi-layer membrane with PP or GF prefilter extends life when filtering samples with high particulate loads
Ultrasonic welding sealing avoids adhesive residues, reducing extractables in sensitive HPLC and GC methods
Available in 4 mm, 13 mm, and 25 mm diameters to match sample volume and particle load requirements
Nine membrane types cover aqueous, organic, and protein-based samples, reducing the need for multiple filter types
Part number and membrane type printed directly on housing for immediate identification at the bench

Multi-layer membrane structure with PP or GF prefilter for extended service life
Ultrasonic welding sealing technology for leak-free performance under pressure
Available in 4 mm, 13 mm, and 25 mm housing diameters
Nine membrane options: Nylon, PTFE, Hydrophilic PTFE, MCE, RC, CA, PES, PVDF, Hydrophilic PVDF, and GF
Pore sizes from 0.1 to 5.0 micron, including 0.45, 0.70, and 1.0 micron for coarse filtration
Maximum operating pressure of 6 bar at 23 C
Female luer lock inlet and male luer slip outlet for standard syringe compatibility
Filtration areas of 0.125 cm² (4 mm), 1.09 cm² (13 mm), and 4.08 cm² (25 mm)

Where It Is Used

Applications And Buyers

Application Scenarios

Filtering cell culture supernatants and protein samples with high particulate content in biotechnology labs
Preparing environmental water samples with suspended solids for ion chromatography or trace metal analysis
Cleaning up crude extracts and fermentation broths in pharmaceutical R&D before HPLC injection
Handling food and beverage samples with high particulate matter, such as fruit pulp or dairy residues
Replacing Whatman Puradisc filters in high-throughput QC labs where filter longevity is critical

Who It Is For

Pharmaceutical R&D labs processing complex biological samples with high particulate content
Environmental testing labs handling soil leachates, sludge, or industrial effluents
Food and beverage labs testing products with suspended solids or high lipid content
Contract testing organizations that require consistent filter performance across multiple sample types
Lab distributors seeking a cost-effective, high-life filter alternative to branded prefiltered syringe filters

Problems It Solves

Filters clogging too quickly when processing samples with high particulate loads
Column frits blocking due to undetected particulates in unfiltered samples
Extractables from solvent-bonded seals or adhesive layers appearing in HPLC baselines
Analysts misidentifying membrane type due to unmarked or poorly labelled housings
High consumable cost from frequent filter changes in dirty sample matrices

Data

Specifications

Filter Media	Nylon, PTFE, Hydrophilic PTFE, MCE, RC, CA, PES, PVDF, Hydrophilic PVDF, GF
Housing	PP
Diameter	4 mm, 13 mm, 25 mm
Filtration Area	0.125 cm² (4 mm), 1.09 cm² (13 mm), 4.08 cm² (25 mm)
Pore Size	0.1, 0.22, 0.45, 0.70, 1.0, 3.0, 5.0 micron
Sealing Technology	Ultrasonic welding
Max Operating Pressure	6 bar at 23 C
Inlet / Outlet	Female inlet lock / male outlet slip
Packing	100 pcs per pack

Materials

Membrane selected per application: Nylon, PTFE, Hydrophilic PTFE, MCE, RC, CA, PES, PVDF, Hydrophilic PVDF, or GF
PP or GF prefilter layer in multi-layer membrane structure

Customization Options

Choice of nine membrane types across 0.1 to 5.0 micron pore sizes
Three housing diameters: 4 mm, 13 mm, 25 mm
OEM printing and private-label packing available through the Microlab OEM division
OEM contract manufacturing and membrane die-cut and packing services offered

How It Is Made

Manufacturing And Quality Control

Manufacturing Process

Membrane die cutting for precise prefilter and main filter layers
Ultrasonic welding to seal membrane into housing
Part number and membrane type printed directly on housing
Packing in 100-piece units with clear labelling

Quality Control

Membrane type and pore size verified against part number before packing
Sealing integrity tested to 6 bar at 23 C
Filtration area measured per diameter to ensure consistency
Visual inspection of housing and membrane alignment before packing

Comparison

Compared With The Alternatives

Versus Single-layer syringe filters

The multi-layer structure with prefilter extends life in dirty samples, reducing the frequency of filter changes and downtime.

Versus Solvent-bonded filters

Ultrasonic welding avoids adhesive in the flow path, which reduces extractables and is critical for sensitive chromatographic methods.

Versus Whatman Puradisc filters

BioFil™ is specified as a direct replacement for Puradisc filters in the same diameters and membrane types, with comparable performance and lower cost.

Versus Reusable filter holders

A single-use filter eliminates carry-over risk and cleaning validation burden, especially important in biotech and pharmaceutical workflows.

Use Guide

How To Use It

Select the membrane based on sample chemistry: PTFE and PP for aggressive solvents, PES and MCE for aqueous samples, Nylon and RC for mixed phases
Use 4 mm for small volumes (under 1 mL) with moderate particulates; 13 mm for standard volumes; 25 mm for high-volume or high-particulate samples
Choose 0.45 micron for routine HPLC clean-up; 0.22 micron for sterile filtration; 0.70 and 1.0 micron for coarse prefiltration
Attach syringe to female luer lock inlet, expel air, and filter at a steady rate
Discard the first few drops if the method is sensitive to wetting solvent
Do not exceed 6 bar at 23 C; if pressure rises rapidly, switch to a larger diameter or coarser pore size

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FAQ

Questions Buyers Ask

What is the difference between BioFil and Easyfil filters?

BioFil has a multi-layer structure with a PP or GF prefilter for high-particulate samples. Easyfil uses a single-layer membrane for low extractables in routine HPLC and GC applications.

Which membrane should I use for protein samples?

Use PES, MCE, or RC membranes for protein samples. Hydrophilic PTFE and hydrophilic PVDF are also suitable for protein-containing matrices.

Can I use the 4 mm filter for large sample volumes?

No. The 4 mm filter has a small filtration area (0.125 cm²) and is intended for small volumes under 1 mL. Use 13 mm or 25 mm for larger volumes.

What is the prefilter made of?

The prefilter is either PP or GF (glass fibre), depending on the part number. GF is used in filters with higher particulate retention capacity.

Is the filter compatible with HPLC systems?

Yes. The ultrasonic welding sealing and low extractables make it suitable for HPLC and GC applications, including gradient runs.

Do you offer private-label packaging?

Yes. The Microlab OEM division supports private-label printing and packing for filter brands and distributors.