Organic Copolymer Base SPE Column product photo 1

SPE Column

Organic Copolymer Base SPE Column

Organic Copolymer Base SPE Columns are designed for the extraction of non-polar to moderately polar acidic, neutral, and basic compounds from complex matrices such as blood, urine, food, and environmental samples. The columns use polymeric sorbents with a hydrophilic-lipophilic balanced (HLB) structure or mixed-mode ion exchange mechanisms, offering high stability across pH 1 to 14 and compatibility with most common solvents. They are suitable for high recovery, reproducible results, and high loadability in routine and method development workflows.

Stable from pH 1 to 14, enabling use across a wide range of sample preparation conditions
3 to 10 times higher adsorption capacity and loadability than C18-bonded silica gel
High surface area of 600 m²/g ensures efficient analyte capture
Monodisperse 40 µm particles reduce band broadening and improve reproducibility

Key Benefits

What This Product Gives You

Stable from pH 1 to 14, enabling use across a wide range of sample preparation conditions
3 to 10 times higher adsorption capacity and loadability than C18-bonded silica gel
High surface area of 600 m²/g ensures efficient analyte capture
Monodisperse 40 µm particles reduce band broadening and improve reproducibility
No bed dryness issues due to high wettability, minimizing breakthrough risk
Compatible with LC-MS/MS and other sensitive detection methods

Sorbent types: HLB, MAX, MCX, WAX, WCX - each tailored for specific compound classes
Particle size: 40 µm, monodisperse for consistent flow and performance
Pore size: 300 Å, enabling access to larger molecules and complex matrices
Surface area: 600 m²/g, supporting high adsorption capacity
Stable across pH 1 to 14, compatible with most organic and aqueous solvents
Available in 3 ml, 6 ml, 12 ml, and 20 ml column volumes
High wettability prevents bed dryness and reduces breakthrough risk

Where It Is Used

Applications And Buyers

Application Scenarios

Extracting drugs and metabolites from blood and urine samples in forensic and clinical testing
Residue analysis of antibiotics, pesticides, and mycotoxins in food and animal products
Cleanup and concentration of veterinary drug residues in milk and meat for export compliance
Environmental monitoring of perfluorinated acids and other strong acids in water samples
Method development for new drug discovery involving strong acids or bases

Who It Is For

Pharmaceutical and clinical laboratories conducting drug metabolism and residue testing
Food safety labs testing for veterinary drug and pesticide residues in animal-origin products
Environmental testing labs analyzing pollutants in water and soil
Contract research organizations requiring reproducible, high-recovery SPE workflows
OEM partners developing standardized sample prep kits using polymeric sorbents

Problems It Solves

Low recovery and poor reproducibility when using silica-based sorbents in extreme pH conditions
Column breakthrough due to insufficient capacity when processing high-volume or high-concentration samples
Bed dryness during SPE procedures leading to analyte loss and inconsistent results
Inadequate retention of basic or acidic compounds on non-mixed-mode sorbents
Method transfer issues caused by variability in sorbent performance across batches

Data

Specifications

Sorbent Type	HLB, MAX, MCX, WAX, WCX
Surface Area	600 m²/g
Particle Size	40 µm
Pore Size	300 Å
pH Stability	1 to 14
Column Volume	3 ml, 6 ml, 12 ml, 20 ml
Mass Options	60mg, 150mg, 200mg, 500mg, 1000mg
Packing	50 pcs/pk (3ml), 30 pcs/pk (6ml), 20 pcs/pk (12ml, 20ml)

Materials

Monodisperse N-vinylpyrrolidone-divinylbenzene copolymer resin for HLB
Quaternary ammonium group bonded copolymer for MAX
Sulfonic acid group bonded polystyrene-divinylbenzene for MCX
Microporous polystyrene-divinylbenzene grafted with amine groups for WAX
Microporous polystyrene-divinylbenzene grafted with carboxylic acid groups for WCX

Customization Options

OEM private-label packaging available through the Microlab OEM division
Custom sorbent formulations and column volumes can be developed for specific applications
Private-label printing and packaging options for distributor and lab brand programs
Support for contract manufacturing of SPE columns for OEM partners

How It Is Made

Manufacturing And Quality Control

Manufacturing Process

Polymer resin synthesis and particle size control
Monodisperse particle formation via suspension polymerization
Functional group grafting for specific retention mechanisms
Column packing with controlled bed density and uniformity
Quality inspection of bed integrity and flow characteristics
Packaging in 50, 30, or 20 units per pack based on volume

Quality Control

Particle size distribution verified to ensure consistency
Surface area measured to confirm 600 m²/g specification
Bed integrity tested under flow to prevent channeling
Packing uniformity checked to avoid breakthrough
Batch-to-batch reproducibility verified for recovery and capacity

Comparison

Compared With The Alternatives

Versus C18-bonded silica gel

The organic copolymer base offers 3 to 10 times higher loadability and is stable from pH 1 to 14, unlike silica which degrades at extreme pH

Versus Silica-based mixed-mode sorbents

Polymeric sorbents maintain structural integrity across pH 1-14 and resist hydrolysis, reducing batch variability

Versus Non-mixed-mode sorbents

Mixed-mode mechanisms (e.g., MCX, MAX) provide superior retention for basic and acidic compounds compared to single-mode resins

Versus Pre-packed silica columns

Polymeric columns show higher recovery and reproducibility in complex matrices like blood and urine due to better chemical stability

Use Guide

How To Use It

Select HLB for broad-spectrum extraction of non-polar to moderately polar compounds
Use MAX for acidic compounds with strong anion exchange and reversed-phase retention
Choose MCX for basic compounds with cation exchange and reversed-phase interaction
Use WAX for strong acids and weak anion exchange applications
Select WCX for strong bases and weak cation exchange, especially quaternary ammonium compounds
Condition the column with 3-5 column volumes of methanol, then equilibrate with water or buffer
Load sample at a controlled flow rate to prevent bed disruption
Elute with appropriate solvent based on analyte polarity and sorbent type
Dry the column before storage if not used immediately

Photos

Product Gallery

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Related Industries And Guides

Environmental Testing Laboratories Food and Beverage Testing Chemical Analysis Laboratories SPE Column Selection Guide

FAQ

Questions Buyers Ask

What is the difference between HLB and MCX sorbents?

HLB is a hydrophilic-lipophilic balanced sorbent suitable for non-polar to moderately polar compounds. MCX is a mixed-mode cation exchange sorbent designed specifically for basic compounds and strong cation exchange.

Can these columns be used for LC-MS/MS analysis?

Yes. The high recovery, low background, and compatibility with common solvents make them suitable for LC-MS/MS workflows.

What is the maximum pH range for these columns?

All columns are stable from pH 1 to 14, allowing use in both acidic and basic sample preparation conditions.

Are the columns compatible with methanol and acetonitrile?

Yes. The organic copolymer base is compatible with most common solvents including methanol, acetonitrile, and water.

How many columns are in a pack?

Packs contain 50 for 3 ml, 30 for 6 ml, and 20 for 12 ml and 20 ml columns.

Can I get these under my own brand?

Yes. The Microlab OEM division offers private-label packaging, printing, and contract manufacturing for SPE columns.