Labware Products

Cell Culture Flask

Cell Culture Flasks are designed for adherent cell culture with a tissue culture-treated surface that supports cell attachment and growth. Available in multiple sizes from 25 cm² to 3750 cm², they feature either vented or plug-seal caps to control gas exchange and prevent contamination. The large mouth design allows easy access for pipetting and scraping, while the smooth, uniform surface enables clear microscopic observation. Sterilized by gamma radiation and manufactured in a dust-free environment, these flasks are non-pyrogenic and free from DNase and RNase.

Tissue culture-treated surface promotes consistent cell adhesion and proliferation for adherent cell lines
Large mouth design simplifies pipetting, cell harvesting, and microscopic inspection
Gamma radiation sterilization ensures non-pyrogenic and DNase/RNase-free performance
Vented caps allow gas exchange without water absorption, reducing contamination risk

Key Benefits

What This Product Gives You

Tissue culture-treated surface promotes consistent cell adhesion and proliferation for adherent cell lines
Large mouth design simplifies pipetting, cell harvesting, and microscopic inspection
Gamma radiation sterilization ensures non-pyrogenic and DNase/RNase-free performance
Vented caps allow gas exchange without water absorption, reducing contamination risk
Smooth, uniform surface provides clear visual monitoring under the microscope
Available in multiple sizes and cap types to match different workflow needs

Tissue culture-treated polystyrene surface for enhanced cell adhesion
Available in 25 cm², 75 cm², 150 cm², 175 cm², 255 cm², and 5-layer 3750 cm² culture areas
Cap options: vented cap for gas exchange or plug seal cap for airtight storage
Large mouth design for easy pipetting and cell scraping
Gamma radiation sterilized, non-pyrogenic, DNase and RNase free
Manufactured in a dust-free environment with controlled particulate levels
Smooth, uniform surface for clear microscopic visualization

Where It Is Used

Applications And Buyers

Application Scenarios

Routine culture of adherent cell lines such as fibroblasts, epithelial cells, and stem cells
Scaling up cell culture from 25 cm² to 3750 cm² for bioproduction or process development
Microscopic monitoring of cell morphology and confluency during culture cycles
Handling of sensitive cell types where contamination and extractables must be minimized
Use in laboratories requiring consistent, validated culture conditions across multiple experiments

Who It Is For

Life-science research labs conducting adherent cell culture for drug screening and basic research
Biopharmaceutical process development teams scaling up cell culture processes
Contract testing labs requiring consistent, non-pyrogenic cultureware for assay validation
Academic and industrial labs using sensitive cell lines where contamination and enzyme activity must be avoided
Lab distributors sourcing validated, sterilized cultureware with consistent surface treatment

Problems It Solves

Poor cell attachment due to untreated or inconsistent surface coatings on cultureware
Contamination from airborne microbes entering through non-vented or poorly sealed caps
Difficulty in harvesting cells due to narrow necks or poor access in standard flasks
Visual interference from surface defects or roughness during microscopic observation
Risk of pyrogens or nucleases affecting cell viability and assay results in sensitive applications

Data

Specifications

Culture Area	25 cm², 75 cm², 150 cm², 175 cm², 255 cm², 3750 cm² (5 layers × 175 cm²)
Capacity	50 ml, 250 ml, 600 ml, 750 ml, 950 ml, 3750 ml
Cap Type	Vent Cap, Plug Seal Cap
Mouth Design	Large mouth for easy access
Sterilization	Gamma radiation
Pyrogen Status	Non-pyrogenic
Nuclease Status	DNase and RNase free
Surface Treatment	Tissue culture treated
Manufacturing Environment	Dust-free manufacturing site
Packing	10 pcs per pack (FK25V, FK25P), 5 pcs per pack (FK75V, FK75P, FK150V, FK150P, FK175V, FK175P, FK255V, FK255P), 3 pcs per pack (FK2551V), 1 pc per pack (FKL175V, FKL175P), 200 packs per case (FK25V, FK25P), 100 packs per case (FK75V, FK75P), 30 packs per case (FK150V, FK150P, FK175V, FK175P), 25 packs per case (FK255V, FK255P), 18 packs per case (FK2551V), 8 packs per case (FKL175V, FKL175P)

Materials

Medical grade USP CLASS VI polystyrene
Polystyrene housing with tissue culture surface treatment

Customization Options

OEM private-label printing available through the Microlab OEM division
Custom packing configurations (packs per case, pieces per pack) can be arranged
Cap type selection (vented or plug seal) available per product line
OEM contract manufacturing and packaging services offered for filter brands

How It Is Made

Manufacturing And Quality Control

Manufacturing Process

Surface treatment for tissue culture adherence
Gamma radiation sterilization
Assembly with vented or plug-seal caps
Inspection under dust-free conditions
Packing according to specified quantities per pack and case

Quality Control

Surface uniformity and treatment verified before packaging
Sterilization validation confirmed via gamma radiation dosimetry
Cap sealing integrity tested to prevent microbial ingress
Packing quantity checked against order specifications
Visual inspection for surface defects and particulates

Comparison

Compared With The Alternatives

Versus Standard polystyrene flasks without tissue culture treatment

The tissue culture-treated surface ensures consistent cell attachment and growth, reducing variability in cell culture results.

Versus Flasks with standard caps

The hydrophobic vent cap prevents water absorption and microbial ingress while allowing gas exchange, improving culture stability.

Versus Small-mouth flasks

The large mouth design enables easier pipetting and cell scraping, reducing handling time and risk of cell damage.

Versus Non-sterilized flasks

Gamma radiation sterilization ensures non-pyrogenic and DNase/RNase-free performance, critical for sensitive cell cultures.

Use Guide

How To Use It

Use tissue culture-treated flasks for adherent cell lines only; avoid for suspension cultures
Select cap type based on culture needs: vented cap for routine culture, plug seal cap for storage or transport
Ensure the flask is sterile and intact before use; do not use if damaged or compromised
Use aseptic technique during media changes and cell harvesting
For microscopic observation, clean the outside of the flask to avoid interference
Store flasks in a dry, clean environment; avoid exposure to UV light or extreme temperatures

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FAQ

Questions Buyers Ask

What is the surface treatment for these flasks?

The flasks have a tissue culture-treated polystyrene surface that promotes cell adhesion and growth for adherent cell lines.

Are these flasks sterilized?

Yes, all flasks are sterilized by gamma radiation and are non-pyrogenic, DNase and RNase free.

What is the difference between vented and plug-seal caps?

Vented caps allow gas exchange during culture while preventing microbial contamination. Plug-seal caps are airtight and used for storage or transport.

Can I use these flasks for suspension cells?

No, these flasks are designed for adherent cell culture. Suspension cells require different vessel types.

What is the packing quantity for each model?

Packing varies: 10 per pack (FK25V, FK25P), 5 per pack (FK75V, FK75P, FK150V, FK150P, FK175V, FK175P, FK255V, FK255P), 3 per pack (FK2551V), 1 per pack (FKL175V, FKL175P), with 200, 100, 30, 25, 18, or 8 packs per case depending on model.

Do you offer private-label packaging?

Yes, the Microlab OEM division provides private-label printing and custom packing options for lab distributors and OEM brands.